Oscillation of mRNA level and activity of granule-bound starch synthaseI in Arabidopsis leaves during the day/night cycle

Oscillation of mRNA level and activity of granule-bound starch synthaseI in Arabidopsis leaves... Granule-bound starch synthase (GBSSI) is one of the most extensively studied enzymes of the starch synthesis pathway and its role in the synthesis of amylose has been well established. However, few studies have been carried out to characterize the regulation of GBSSI gene. Regulation of starch synthesis genes is especially interesting in photosynthetic tissues, where starch is subjected to a periodical alternation of synthesis and degradation during the day/night cycle. In this report we show a circadian oscillation of GBSSI mRNA levels in leaves of Arabidopsis during the day/night cycle, and provide evidence that GBSSI expression is controlled by the transcription factors CCA1 and LHY. Over-expression of both CCA1 and LHY genes causes the elimination of GBSSI mRNA oscillation. Binding shift assays indicate that this control may be exerted through a direct interaction of those regulatory proteins with the GBSSI promoter. Oscillation is not observed on the GBSSI protein levels, which remains constant along the cycle. However, GBSSI activity shows a clear oscillation with a period of 24 h that is altered in transgenic plants over-expressing CCA1. Possible mechanisms controlling GBSSI activity during the day/night cycle are discussed. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

Oscillation of mRNA level and activity of granule-bound starch synthaseI in Arabidopsis leaves during the day/night cycle

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Publisher
Kluwer Academic Publishers
Copyright
Copyright © 2003 by Kluwer Academic Publishers
Subject
Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1023/A:1023053420632
Publisher site
See Article on Publisher Site

Abstract

Granule-bound starch synthase (GBSSI) is one of the most extensively studied enzymes of the starch synthesis pathway and its role in the synthesis of amylose has been well established. However, few studies have been carried out to characterize the regulation of GBSSI gene. Regulation of starch synthesis genes is especially interesting in photosynthetic tissues, where starch is subjected to a periodical alternation of synthesis and degradation during the day/night cycle. In this report we show a circadian oscillation of GBSSI mRNA levels in leaves of Arabidopsis during the day/night cycle, and provide evidence that GBSSI expression is controlled by the transcription factors CCA1 and LHY. Over-expression of both CCA1 and LHY genes causes the elimination of GBSSI mRNA oscillation. Binding shift assays indicate that this control may be exerted through a direct interaction of those regulatory proteins with the GBSSI promoter. Oscillation is not observed on the GBSSI protein levels, which remains constant along the cycle. However, GBSSI activity shows a clear oscillation with a period of 24 h that is altered in transgenic plants over-expressing CCA1. Possible mechanisms controlling GBSSI activity during the day/night cycle are discussed.

Journal

Plant Molecular BiologySpringer Journals

Published: Oct 7, 2004

References

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