Mammalian Genome 8, 148-152 (1997). 9 Spnnger-Verlag New York Inc 1997 M. Rijnkels, 1'2'* P.M. Kooiman, 2 H.A. de Boer 1 F.R. Pieper 2 'Medical Biotechnology Department, Leiden Institute of Chemistry, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands 2pharming B.V., Niels Bohrweg 11-13, 2333 CA Leiden, The Netherlands Received: 15 July 1996 / Accepted: 13 October 1996 In bovine milk, four types of caseins (O~s~-, cts2-, 13-, and K-casein) buffer, including 0.1 mg/ml BSA and 2 mM spermidine, and 20--60 have been identified, each encoded by a single copy gene. For each units of restriction enzyme were added. The restriction enzyme of these, several genetic variants have been described. The casein was allowed to diffuse into the agarose block for 1 h on ice. genes are clustered in a region on Chromosome (Chr) 6 (Ferretti et Digestions were performed overnight at the appropriate tempera- al. 1990; Threadgill and Womack 1990; Gallagher et al. 1994), and tures. Double digestions were performed sequentially after equili- their overall organization is conserved between species (Mercier bration to the second digestion buffer. and Vilotte 1993). Pulsed-field gel electrophoresis was carried out with a contour The three genes encoding the calcium-sensitive caseins
Mammalian Genome – Springer Journals
Published: Mar 24, 2009
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