Oral and intraperitoneal immunization with rotavirus 2/6 virus-like particles stimulates a systemic and mucosal immune response in mice

Oral and intraperitoneal immunization with rotavirus 2/6 virus-like particles stimulates a... We examined the use of a virus-like particle (VLP) as an immunogen by analysing the IgA and IgG response generated in serum, intestinal (fecal), pulmonary and uterine samples. The particle comprised two rotavirus capsid proteins (simian VP2 and murine VP6) generated using recombinant baculovirus expression of the two capsid proteins, which self-assembled into particulate VLP2/6. Mice were immunized orally or intraperitoneally (i.p.) with 0 or 100 µg VLP2/6 with or without 5 µg cholera toxin adjuvant. The results showed a systemic and mucosal immune response to VLP2/6 when administered i.p. and, to a lesser extent, when delivered orally which was not dependant on adjuvant use and further proves the concept of VLP2/6 as an effective immunogen. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Oral and intraperitoneal immunization with rotavirus 2/6 virus-like particles stimulates a systemic and mucosal immune response in mice

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Publisher
Springer Journals
Copyright
Copyright © 2005 by Springer-Verlag/Wien
Subject
Biomedicine; Medical Microbiology; Virology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-004-0447-z
Publisher site
See Article on Publisher Site

Abstract

We examined the use of a virus-like particle (VLP) as an immunogen by analysing the IgA and IgG response generated in serum, intestinal (fecal), pulmonary and uterine samples. The particle comprised two rotavirus capsid proteins (simian VP2 and murine VP6) generated using recombinant baculovirus expression of the two capsid proteins, which self-assembled into particulate VLP2/6. Mice were immunized orally or intraperitoneally (i.p.) with 0 or 100 µg VLP2/6 with or without 5 µg cholera toxin adjuvant. The results showed a systemic and mucosal immune response to VLP2/6 when administered i.p. and, to a lesser extent, when delivered orally which was not dependant on adjuvant use and further proves the concept of VLP2/6 as an effective immunogen.

Journal

Archives of VirologySpringer Journals

Published: Feb 1, 2005

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