Optimization of DNA extraction in the spiny spider crab Maja brachydactyla: determining the best extraction method, sample conservation and starting tissue

Optimization of DNA extraction in the spiny spider crab Maja brachydactyla: determining the best... Methodological studies are an important previous step in molecular research. We optimize a method for DNA extraction from eggs and a noninvasive extraction method for adult tissues of Maja brachydactyla. Our results show that lyophilized eggs in B developmental stage are the most suitable for DNA extraction. Regarding adult tissues, we propose setae as an alternative tissue for DNA extraction, since prevents mutilation of specimens, which avoids infections and fitness reduction. That is especially relevant in studies with endangered species of crustaceans. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Conservation Genetics Resources Springer Journals

Optimization of DNA extraction in the spiny spider crab Maja brachydactyla: determining the best extraction method, sample conservation and starting tissue

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Publisher
Springer Netherlands
Copyright
Copyright © 2017 by Springer Science+Business Media Dordrecht
Subject
Life Sciences; Conservation Biology/Ecology; Ecology; Biodiversity; Evolutionary Biology; Plant Genetics and Genomics; Animal Genetics and Genomics
eISSN
1877-7260
D.O.I.
10.1007/s12686-017-0685-5
Publisher site
See Article on Publisher Site

Abstract

Methodological studies are an important previous step in molecular research. We optimize a method for DNA extraction from eggs and a noninvasive extraction method for adult tissues of Maja brachydactyla. Our results show that lyophilized eggs in B developmental stage are the most suitable for DNA extraction. Regarding adult tissues, we propose setae as an alternative tissue for DNA extraction, since prevents mutilation of specimens, which avoids infections and fitness reduction. That is especially relevant in studies with endangered species of crustaceans.

Journal

Conservation Genetics ResourcesSpringer Journals

Published: Jan 20, 2017

References

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