Optimization of balloon-type bubble bioreactor angle and methyl jasmonate concentration to enhance metabolite production in adventitious roots of Pseudostellaria heterophylla

Optimization of balloon-type bubble bioreactor angle and methyl jasmonate concentration to... Adventitious roots of Pseudostellaria heterophylla were cultivated in 3-L balloon-type bubble bioreactors (BTBB). The optimum angle of the base of the bioreactor was 90°. The rate of growth (64.96) and electrical conductivity (2.3 μS cm−1) were highest in the culture with the 90° bioreactor angle, and total saponins (9.61 mg g−1), polysaccharides (128.29 mg g−1) and amino acids (0.25 mg g−1) content were also maximum. We also investigated the effects of a single elicitor (methyl jasmonate; MJ) on accumulation of metabolites in P. heterophylla adventitious root culture in 3-L BTBB. The highest rate of growth (21.4) was achieved in the control group, in which the highest amino acids yield was 1.39 mg L−1. The highest total saponins (20.98 mg L−1) and polysaccharides (1.18 mg L−1) yields were obtained after addition of 10 mg L−1 MJ, and were, respectively, 1.28 and 1.23-fold higher than in the control group. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Research on Chemical Intermediates Springer Journals

Optimization of balloon-type bubble bioreactor angle and methyl jasmonate concentration to enhance metabolite production in adventitious roots of Pseudostellaria heterophylla

Loading next page...
 
/lp/springer_journal/optimization-of-balloon-type-bubble-bioreactor-angle-and-methyl-VxkHhJpoMq
Publisher
Springer Netherlands
Copyright
Copyright © 2014 by Springer Science+Business Media Dordrecht
Subject
Chemistry; Catalysis; Physical Chemistry; Inorganic Chemistry
ISSN
0922-6168
eISSN
1568-5675
D.O.I.
10.1007/s11164-014-1681-y
Publisher site
See Article on Publisher Site

Abstract

Adventitious roots of Pseudostellaria heterophylla were cultivated in 3-L balloon-type bubble bioreactors (BTBB). The optimum angle of the base of the bioreactor was 90°. The rate of growth (64.96) and electrical conductivity (2.3 μS cm−1) were highest in the culture with the 90° bioreactor angle, and total saponins (9.61 mg g−1), polysaccharides (128.29 mg g−1) and amino acids (0.25 mg g−1) content were also maximum. We also investigated the effects of a single elicitor (methyl jasmonate; MJ) on accumulation of metabolites in P. heterophylla adventitious root culture in 3-L BTBB. The highest rate of growth (21.4) was achieved in the control group, in which the highest amino acids yield was 1.39 mg L−1. The highest total saponins (20.98 mg L−1) and polysaccharides (1.18 mg L−1) yields were obtained after addition of 10 mg L−1 MJ, and were, respectively, 1.28 and 1.23-fold higher than in the control group.

Journal

Research on Chemical IntermediatesSpringer Journals

Published: May 21, 2014

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off