Optimization of balloon-type bubble bioreactor angle and methyl jasmonate concentration to enhance metabolite production in adventitious roots of Pseudostellaria heterophylla

Optimization of balloon-type bubble bioreactor angle and methyl jasmonate concentration to... Adventitious roots of Pseudostellaria heterophylla were cultivated in 3-L balloon-type bubble bioreactors (BTBB). The optimum angle of the base of the bioreactor was 90°. The rate of growth (64.96) and electrical conductivity (2.3 μS cm−1) were highest in the culture with the 90° bioreactor angle, and total saponins (9.61 mg g−1), polysaccharides (128.29 mg g−1) and amino acids (0.25 mg g−1) content were also maximum. We also investigated the effects of a single elicitor (methyl jasmonate; MJ) on accumulation of metabolites in P. heterophylla adventitious root culture in 3-L BTBB. The highest rate of growth (21.4) was achieved in the control group, in which the highest amino acids yield was 1.39 mg L−1. The highest total saponins (20.98 mg L−1) and polysaccharides (1.18 mg L−1) yields were obtained after addition of 10 mg L−1 MJ, and were, respectively, 1.28 and 1.23-fold higher than in the control group. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Research on Chemical Intermediates Springer Journals

Optimization of balloon-type bubble bioreactor angle and methyl jasmonate concentration to enhance metabolite production in adventitious roots of Pseudostellaria heterophylla

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Publisher
Springer Netherlands
Copyright
Copyright © 2014 by Springer Science+Business Media Dordrecht
Subject
Chemistry; Catalysis; Physical Chemistry; Inorganic Chemistry
ISSN
0922-6168
eISSN
1568-5675
D.O.I.
10.1007/s11164-014-1681-y
Publisher site
See Article on Publisher Site

Abstract

Adventitious roots of Pseudostellaria heterophylla were cultivated in 3-L balloon-type bubble bioreactors (BTBB). The optimum angle of the base of the bioreactor was 90°. The rate of growth (64.96) and electrical conductivity (2.3 μS cm−1) were highest in the culture with the 90° bioreactor angle, and total saponins (9.61 mg g−1), polysaccharides (128.29 mg g−1) and amino acids (0.25 mg g−1) content were also maximum. We also investigated the effects of a single elicitor (methyl jasmonate; MJ) on accumulation of metabolites in P. heterophylla adventitious root culture in 3-L BTBB. The highest rate of growth (21.4) was achieved in the control group, in which the highest amino acids yield was 1.39 mg L−1. The highest total saponins (20.98 mg L−1) and polysaccharides (1.18 mg L−1) yields were obtained after addition of 10 mg L−1 MJ, and were, respectively, 1.28 and 1.23-fold higher than in the control group.

Journal

Research on Chemical IntermediatesSpringer Journals

Published: May 21, 2014

References

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