Adventitious roots of Pseudostellaria heterophylla were cultivated in 3-L balloon-type bubble bioreactors (BTBB). The optimum angle of the base of the bioreactor was 90°. The rate of growth (64.96) and electrical conductivity (2.3 μS cm−1) were highest in the culture with the 90° bioreactor angle, and total saponins (9.61 mg g−1), polysaccharides (128.29 mg g−1) and amino acids (0.25 mg g−1) content were also maximum. We also investigated the effects of a single elicitor (methyl jasmonate; MJ) on accumulation of metabolites in P. heterophylla adventitious root culture in 3-L BTBB. The highest rate of growth (21.4) was achieved in the control group, in which the highest amino acids yield was 1.39 mg L−1. The highest total saponins (20.98 mg L−1) and polysaccharides (1.18 mg L−1) yields were obtained after addition of 10 mg L−1 MJ, and were, respectively, 1.28 and 1.23-fold higher than in the control group.
Research on Chemical Intermediates – Springer Journals
Published: May 21, 2014
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