The method for genetic transformation of maize (Zea mays L.) via embryogenic callus infection with Agrobacterium tumefaciens was developed. Calli were co-cultivated with the overnight culture of A. tumefaciens strain LBA4404 harboring the pBI121 plasmid with the nptII and uidA genes. Thereafter, the sensitivity of calli and regenerated plantlets to kanamycin (Km) was determined. It was shown that kanamycin selection was more efficient at the stage of regenerated plantlets than in callus culture. Both vacuum infiltration at the infection step and preliminary activation of Agrobacterium by acetosyringone or by tobacco leaves exudate increased the frequency of Km-resistant plants. The frequency of Km-resistant plants also varied depending on the morphogenic ability of calli. Polymerase chain reaction confirmed the presence of the nptII gene in the genome of regenerated plants and their progeny. β-Glucuronidase gene expression was observed in roots of T1 plants.
Russian Journal of Plant Physiology – Springer Journals
Published: Aug 9, 2005
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
All the latest content is available, no embargo periods.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud