Optimization and application of real-time PCR method for detecting the expression levels of nitrogen assimilation-related genes in rice

Optimization and application of real-time PCR method for detecting the expression levels of... By optimizing the Mg2+ concentration, Taq enzyme dosage, SYBR Green I (SGI) concentration, and plate reading temperature in PCR system, we established the method for detecting the expression levels of nitrogen assimilation-related genes in rice by using RT-PCR technique. Based on this qualified method, we investigated the variations of OsAMT1.1 (one of nitrogen uptake genes) and OsGlt1 (one of nitrogen metabolism genes) expression levels in rice seedlings under conditions of varying nitrogen supply. The results show that by optimizing the parameters in the PCR system to fit the characters of target genes best, we can successfully quantify the low-abundant nitrogen transport and metabolism genes in rice quickly and exactly using fluorescence RT-PCR technique. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Optimization and application of real-time PCR method for detecting the expression levels of nitrogen assimilation-related genes in rice

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Publisher
Nauka/Interperiodica
Copyright
Copyright © 2006 by MAIK “Nauka/Interperiodica”
Subject
Life Sciences; Plant Sciences; Plant Physiology
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443706040200
Publisher site
See Article on Publisher Site

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