Optically sliced micro-PIV using confocal laser scanning microscopy (CLSM)

Optically sliced micro-PIV using confocal laser scanning microscopy (CLSM) Optically sliced microscopic-particle image velocimetry (micro-PIV) is developed using confocal laser scanning microscopy (CLSM). The developed PIV system shows a unique optical slicing capability allowing true depth-wise resolved micro-PIV vector field mapping. A comparative study between CLSM micro-PIV and conventional epi-fluorescence micro-PIV is presented. Both techniques have been applied to the creeping Poiseuille flows in two different microtubes of 99-μm (Re=0.00275) and 516-μm ID diameters (Re=0.021), which are respectively imaged by a 40×-0.75NA objective with an estimated 2.8-μm optical slice thickness, and by a 10×-0.30NA objective with a 26.7-μm slicing. Compared to conventional micro-PIV, CLSM micro-PIV consistently shows significantly improved particle image contrasts, definitions, and measured flow vector fields agreeing more accurately with predictions based on the Poiseuille flow fields. The data improvement due to the optical slicing of CLSM micro-PIV is more pronounced with higher magnification imaging with higher NA objectives for a smaller microtube. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Experiments in Fluids Springer Journals

Optically sliced micro-PIV using confocal laser scanning microscopy (CLSM)

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Publisher
Springer-Verlag
Copyright
Copyright © 2004 by Springer-Verlag
Subject
Engineering
ISSN
0723-4864
eISSN
1432-1114
D.O.I.
10.1007/s00348-004-0790-6
Publisher site
See Article on Publisher Site

Abstract

Optically sliced microscopic-particle image velocimetry (micro-PIV) is developed using confocal laser scanning microscopy (CLSM). The developed PIV system shows a unique optical slicing capability allowing true depth-wise resolved micro-PIV vector field mapping. A comparative study between CLSM micro-PIV and conventional epi-fluorescence micro-PIV is presented. Both techniques have been applied to the creeping Poiseuille flows in two different microtubes of 99-μm (Re=0.00275) and 516-μm ID diameters (Re=0.021), which are respectively imaged by a 40×-0.75NA objective with an estimated 2.8-μm optical slice thickness, and by a 10×-0.30NA objective with a 26.7-μm slicing. Compared to conventional micro-PIV, CLSM micro-PIV consistently shows significantly improved particle image contrasts, definitions, and measured flow vector fields agreeing more accurately with predictions based on the Poiseuille flow fields. The data improvement due to the optical slicing of CLSM micro-PIV is more pronounced with higher magnification imaging with higher NA objectives for a smaller microtube.

Journal

Experiments in FluidsSpringer Journals

Published: Mar 19, 2004

References

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