Optical Recording of Electrical Activity

Optical Recording of Electrical Activity J. Membrane Biol. 208, 89–90 (2005) DOI: 10.1007/s00232-005-0821-0 Thematic Issue B.M. Salzberg, Guest Editor Foreword B.M. Salzberg Departments of Neuroscience and Physiology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA Received Received:: 1122 December December 2005 2005 More than three decades have passed since the of targeting an indicator to a selected subset of cells, introduction of potentiometric dyes for monitoring or to specific regions of cells. These authors also electrical activity in cells and tissues. At its inception, discuss one particularly elegant solution to this quest optical measurement of membrane potential prom- using, as a voltage-sensitive Fo¨ rster Resonance En- ised advantages under circumstances where electrodes ergy Transfer (FRET) acceptor, a highly mobile but were difficult to use for reasons of size, complexity, or non-fluorescent synthetic anion, dipicrylamine. The membrane topology. While many of these benefits genetically targeted fluorescence donor in this hybrid have been realized, what is most remarkable today is scheme is a modified eGFP that can be localized the number of applications that were not, or could exclusively to the inner leaflet of the plasma mem- not have been, anticipated. Many of these are to be brane. found in the papers contained in http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Membrane Biology Springer Journals

Optical Recording of Electrical Activity

Loading next page...
 
/lp/springer_journal/optical-recording-of-electrical-activity-Fu3WMXMZcz
Publisher
Springer-Verlag
Copyright
Copyright © 2006 by Springer Science+Business Media, Inc.
Subject
Life Sciences; Human Physiology; Biochemistry, general
ISSN
0022-2631
eISSN
1432-1424
D.O.I.
10.1007/s00232-005-0821-0
Publisher site
See Article on Publisher Site

Abstract

J. Membrane Biol. 208, 89–90 (2005) DOI: 10.1007/s00232-005-0821-0 Thematic Issue B.M. Salzberg, Guest Editor Foreword B.M. Salzberg Departments of Neuroscience and Physiology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA Received Received:: 1122 December December 2005 2005 More than three decades have passed since the of targeting an indicator to a selected subset of cells, introduction of potentiometric dyes for monitoring or to specific regions of cells. These authors also electrical activity in cells and tissues. At its inception, discuss one particularly elegant solution to this quest optical measurement of membrane potential prom- using, as a voltage-sensitive Fo¨ rster Resonance En- ised advantages under circumstances where electrodes ergy Transfer (FRET) acceptor, a highly mobile but were difficult to use for reasons of size, complexity, or non-fluorescent synthetic anion, dipicrylamine. The membrane topology. While many of these benefits genetically targeted fluorescence donor in this hybrid have been realized, what is most remarkable today is scheme is a modified eGFP that can be localized the number of applications that were not, or could exclusively to the inner leaflet of the plasma mem- not have been, anticipated. Many of these are to be brane. found in the papers contained in

Journal

The Journal of Membrane BiologySpringer Journals

Published: Jan 1, 2005

There are no references for this article.

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off