The role of the prqR gene in the regulation of the adaptive response of the cyanobacteriumSynechocystis sp. PCC6803 to the oxidative stress induced with methyl viologen (MV) was studied. For this, mRNA levels of prqR and the genes, which may be involved in the control of resistance to MV, was determined by means of Northern blot hybridization in wild-type cells and in the MV-resistant Prq20 mutant with a mutation located in the DNA-binding domain of the PrqR protein. It was revealed that the prqR gene belongs to the prqR–prqA operon and downregulates its transcription. In cells of the wild-type strain treated with MV, the autorepressor activity of the PrqR protein enhances and transcript levels of mvrA and sodB genes encoding respectively an assumed transporter protein and iron-containing superoxide dismutase increases significantly. The prqR gene may be involved in the negative, indirect control of transcription of these genes. In this connection, the Prq20 mutant is characterized by an MV-independent derepression of the prqR–prqA operon and by a slightly increased transcript levels of mvrA and sodBgenes not stimulated by MV. Moreover, the expression of mvrA and sodB genes was significantly lower than in wild-type cells after the MV treatment. On the strength of this evidence, it is assumed that the main mechanism underlying for the resistance to MV in the Prq20 mutant is derepression of the prqAgene, the product of which is homologous to multidrug transporters, the drug efflux proteins.
Russian Journal of Genetics – Springer Journals
Published: Oct 7, 2004
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