Arch Virol (1998) 143: 557–562
Nucleotide sequence analysis of the coat protein genes of two
Korean isolates of sweet potato feathery mottle potyvirus*
K. H. Ryu
,and W. M. Park
Department of Plant Pathology, Cornell University, Ithaca, New York, U.S.A.
Graduate School of Biotechnology, Korea University, Seoul, Korea
Accepted November 13, 1997
Summary. The coat protein (CP) genes of the genomic RNA of two Korean
isolates of sweet potato feathery mottle potyvirus (SPFMV), SPFMV-K1 and
SPFMV-K2, were cloned and their complete nucleotide sequences were deter-
mined. Sequence comparisons of the two Korean isolates showed 97.8% amino
acid identity in the CP cistron, and 79.9% to 99.0% identity with those of 6 other
known SPFMV strains. Of 74 amino acid changes totally among the SPFMV
strains, 39 changes were located at the N-terminal region. Pairwise amino acid
sequence comparison revealed sequence similarities of 48.6 to 70.2% between
SPFMV and 20 other potyviruses, indicating SPFMV to be a quite distinct species.
Multiple alignment of the CP cistrons from other potyviruses showed that most
of the conserved amino acid residues of the genus Potyvirus are well preserved
in the corresponding locations.
Sweet potato feathery mottle virus (SPFMV), a species of the genus Potyvirus of
the family Potyviridae, is the most prevalent virus and widespread throughout the
world where sweet potato plants are cultivated [6, 21, 24, 26, 35]. The members
of the genus are transmitted by aphids in a non-persistent manner. The potyviral
genome is a single-stranded, positive sense RNA, and has a poly (A) tract at
termini and a genome-linked protein (VPg) at its 5
termini [15, 17]. The
The nucleotide sequences reported in this paper will appear in the GenBank/EMBL/
DDBJ Nucleotide Sequence Databases under the accession numbers AF015540 (SPFMV-
K1), AF015541 (SPFMV-K2) and AJ001105 (SPFCP5).