Nucleotide polymorphisms of the human papillomavirus 16 E1 gene

Nucleotide polymorphisms of the human papillomavirus 16 E1 gene The E1 ORF is one of the most conserved regions in the human papillomavirus (HPV) genome. The complete E1 gene of the HPV16 genome was amplified with four overlapping primer sets in 16 high-grade (CIN II, III) and 13 low-grade cervical (CIN I) intraepithelial neoplasias as well as in one cervical cancer case. Sequence analysis of the E6 and E7 genes was also carried out in the same cervical samples in order to confirm the association between nucleotide sequence variations in the HPV16 E1 ORF and HPV16 variant lineages. Analysis of the E1 ORF revealed 27 nucleotide changes, and these changes were correlated with those found in HPV16 Asian American and African type II variants. Of these nucleotide variations, A1668G, G2073A, T2169C, T2189C, A2453T, C2454T, A2587T and G2650A were identified only in high-grade dysplasia cases. A phylogenetic tree of the E1 ORF and nucleotide sequence analysis of the E1, E6 and E7 genes revealed that intratypic nucleotide sequence polymorphisms located in the E1 ORF can be used to identify the major phylogenetic branch to which a HPV16 genome belongs. Moreover, amplification of the E1 ORF revealed a disruption between nucleotides 878 and 1523 in five high- and two low-grade cervical cases, indicating that integration of HPV DNA occurs at an early stage of viral infection. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Nucleotide polymorphisms of the human papillomavirus 16 E1 gene

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Publisher
Springer Vienna
Copyright
Copyright © 2014 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-013-1790-8
Publisher site
See Article on Publisher Site

References

  • Molecular and phylogenetic analysis of the HPV 16 E4 gene in cervical lesions from women in Greece
    Tsakogiannis, D; Ruether, IGA; Kyriakopoulou, Z; Pliaka, V; Skordas, V; Gartzonika, C; Levidiotou-Stefanou, S; Markoulatos, P
  • E1 initiator DNA binding specificity is unmasked by selective inhibition of non-specific DNA binding
    Stenlund, A
  • New method for the extraction of viral RNA and DNA from cerebrospinal fluid for use in the polymerase chain reaction assay
    Casas, I; Powell, L; Klapper, PE; Cleator, GM
  • Sequence variation analysis of the E2 gene of human papilloma virus type 16 in cervical lesions from women in Greece
    Tsakogiannis, D; Ruether, IG; Kyriakopoulou, Z; Pliaka, V; Theoharopoulou, A; Skordas, V; Panotopoulou, E; Nepka, C; Markoulatos, P
  • HPV genotyping and HPV16 variant analysis in glandular and squamous neoplastic lesions of the uterine cervix
    Quint, KD; Koning, MN; Doorn, LJ; Quint, WG; Pirog, EC
  • Human papillomavirus (HPV) genotypes and HPV16 variants and risk of adenocarcinoma and squamous cell carcinoma of the cervix
    Tornesello, ML; Losito, S; Benincasa, G; Fulciniti, F; Botti, G; Greggi, S; Buonaguro, L; Buonaguro, FM

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