Nuclear identity specifies transcriptional initiation in plant mitochondria

Nuclear identity specifies transcriptional initiation in plant mitochondria Alterations in mitochondrial gene expression and abnormal floral phenotypes, such as male sterility, characterize alloplasmic plants having the nucleus from Nicotiana tabacum combined with the cytoplasm from Nicotiana repanda. In all Nicotiana species investigated the mitochondrial atp1 gene is co-transcribed with the upstream orf274; however, unique for alloplasmic plants is a marked accumulation of these mitochondrial co-transcripts. In the present work, we show that a major component of the transcript difference is that in the alloplasmic male-sterile plants transcription initiates from novel sites internal to orf274. The sequences surrounding these initiation sites lack the CRTA consensus motif for plant mitochondrial promoters as well as similarity to other known plant mitochondrial promoters. Thus, initiation of transcription is under control of a mitochondrial promoter of a novel non-consensus type. This non-consensus promoter is inactivated when the fertility restoring heritable fragment chromosome from N. repanda is present in the N. tabacum nucleus of the alloplasmic plants. Our data suggest that the fertility-restoring fragment chromosome encodes a factor that represses initiation from this unusual promoter. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

Nuclear identity specifies transcriptional initiation in plant mitochondria

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Publisher
Kluwer Academic Publishers
Copyright
Copyright © 2002 by Kluwer Academic Publishers
Subject
Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1023/A:1014533023003
Publisher site
See Article on Publisher Site

Abstract

Alterations in mitochondrial gene expression and abnormal floral phenotypes, such as male sterility, characterize alloplasmic plants having the nucleus from Nicotiana tabacum combined with the cytoplasm from Nicotiana repanda. In all Nicotiana species investigated the mitochondrial atp1 gene is co-transcribed with the upstream orf274; however, unique for alloplasmic plants is a marked accumulation of these mitochondrial co-transcripts. In the present work, we show that a major component of the transcript difference is that in the alloplasmic male-sterile plants transcription initiates from novel sites internal to orf274. The sequences surrounding these initiation sites lack the CRTA consensus motif for plant mitochondrial promoters as well as similarity to other known plant mitochondrial promoters. Thus, initiation of transcription is under control of a mitochondrial promoter of a novel non-consensus type. This non-consensus promoter is inactivated when the fertility restoring heritable fragment chromosome from N. repanda is present in the N. tabacum nucleus of the alloplasmic plants. Our data suggest that the fertility-restoring fragment chromosome encodes a factor that represses initiation from this unusual promoter.

Journal

Plant Molecular BiologySpringer Journals

Published: Oct 13, 2004

References

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