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Arch Virol (2013) 158:273–275 DOI 10.1007/s00705-012-1444-2 ANNOTATED SEQUENCE RECORD Novel variants of grapevine leafroll-associated virus 4 and 7 detected from a grapevine showing leafroll symptoms • • • Takao Ito Ryoji Nakaune Masaaki Nakano Koichi Suzaki Received: 12 June 2012 / Accepted: 8 July 2012 / Published online: 28 August 2012 Springer-Verlag 2012 All grapevine leafroll-associated viruses (GLRaV) identi- leafroll symptoms. However, each RT-PCR [10] using fied so far belong to the family Closteroviridae [9]. The the GLRaV-1–6-, and -9-specific primer pairs (Table S1; genus Closterovirus includes GLRaV-2, and the genus [2, 4, 8, 10, 12]) was negative. Only RT-PCR [10] using Ampelovirus includes GLRaV-1 and -3 in subgroup I and the GLRaV-7-specific primer pair (Table S1; [6]) GLRaV-4, including the genetically divergent variants detected a faint positive amplification under the follow- GLRaV-5, -6, -9, -De, -Pr and -Car, in subgroup II [9]. The ing PCR conditions: pre-activation at 95 C for 10 min provisional genus ‘‘Velarivirus’’ includes GLRaV-7 [9]. In followed by 43 cycles of denaturation (95 C for 20 s), Japan, although GLRaV-1, -2, and -3 are prevalent, GLRaV-4 primer annealing (42 C for 60 s), and extension (72 C and -7 have not been found. for
Archives of Virology – Springer Journals
Published: Jan 1, 2013
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