Arch Virol (2013) 158:273–275 DOI 10.1007/s00705-012-1444-2 ANNOTATED SEQUENCE RECORD Novel variants of grapevine leafroll-associated virus 4 and 7 detected from a grapevine showing leafroll symptoms • • • Takao Ito Ryoji Nakaune Masaaki Nakano Koichi Suzaki Received: 12 June 2012 / Accepted: 8 July 2012 / Published online: 28 August 2012 Springer-Verlag 2012 All grapevine leafroll-associated viruses (GLRaV) identi- leafroll symptoms. However, each RT-PCR  using ﬁed so far belong to the family Closteroviridae . The the GLRaV-1–6-, and -9-speciﬁc primer pairs (Table S1; genus Closterovirus includes GLRaV-2, and the genus [2, 4, 8, 10, 12]) was negative. Only RT-PCR  using Ampelovirus includes GLRaV-1 and -3 in subgroup I and the GLRaV-7-speciﬁc primer pair (Table S1; ) GLRaV-4, including the genetically divergent variants detected a faint positive ampliﬁcation under the follow- GLRaV-5, -6, -9, -De, -Pr and -Car, in subgroup II . The ing PCR conditions: pre-activation at 95 C for 10 min provisional genus ‘‘Velarivirus’’ includes GLRaV-7 . In followed by 43 cycles of denaturation (95 C for 20 s), Japan, although GLRaV-1, -2, and -3 are prevalent, GLRaV-4 primer annealing (42 C for 60 s), and extension (72 C and -7 have not been found. for
Archives of Virology – Springer Journals
Published: Jan 1, 2013
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera