Plant Mol Biol (2017) 93:197–208 DOI 10.1007/s11103-016-0558-4 Novel rice mutants overexpressing the brassinosteroid catabolic gene CYP734A4 1,2 1,3 1 1 2 Wenjing Qian · Chao Wu · Yaping Fu · Guocheng Hu · Zhengquan He · Wenzhen Liu Received: 17 April 2016 / Accepted: 29 October 2016 / Published online: 4 November 2016 © Springer Science+Business Media Dordrecht 2016 Abstract seed setting rate were also found in heterozygous brd3- Key message Moderate overexpression of CYP734A4 D. To our knowledge, these traits have not been reported improves grain number per main panicle and seed set- in other BR deficient mutants. Quantitative real-time PCR ting rate. analysis indicated that phenotypic severity of the brd3-D Abstract Brassinosteroid (BR) homeostasis and signaling mutant is positively correlated with the CYP734A4 tran- are crucial for plant growth and development. CYP734A scription level. In accordance with the increased expres- genes encode cytochrome P450 monooxygenases that con- sion of CYP734A4, a lower castasterone (a rice BR) content trol the level of bioactive BRs by degrading BRs. How- was detected in the brd3-D mutants. Knockout of brd3-D ever, fertile plants overexpressing CYP734As have not been by using the CRISPR/Cas9 system rescued the mutation. reported in rice. Here, we isolated a novel semi-dominant In addition,
Plant Molecular Biology – Springer Journals
Published: Nov 4, 2016
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