NOD promoter-controlled AtIRT1 expression functions synergistically with NAS and FERRITIN genes to increase iron in rice grains

NOD promoter-controlled AtIRT1 expression functions synergistically with NAS and FERRITIN genes... Rice is a staple food for over half of the world’s population, but it contains only low amounts of bioavailable micronutrients for human nutrition. Consequently, micronutrient deficiency is a widespread health problem among people who depend primarily on rice as their staple food. Iron deficiency anemia is one of the most serious forms of malnutrition. Biofortification of rice grains for increased iron content is an effective strategy to reduce iron deficiency. Unlike other grass species, rice takes up iron as Fe(II) via the IRON REGULATED TRANSPORTER (IRT) in addition to Fe(III)-phytosiderophore chelates. We expressed Arabidopsis IRT1 (AtIRT1) under control of the Medicago sativa EARLY NODULIN 12B promoter in our previously developed high-iron NFP rice lines expressing NICOTIANAMINE SYNTHASE (AtNAS1) and FERRITIN. Transgenic rice lines expressing AtIRT1 alone had significant increases in iron and combined with NAS and FERRITIN increased iron to 9.6 µg/g DW in the polished grains that is 2.2-fold higher as compared to NFP lines. The grains of AtIRT1 lines also accumulated more copper and zinc but not manganese. Our results demonstrate that the concerted expression of AtIRT1, AtNAS1 and PvFERRITIN synergistically increases iron in both polished and unpolished rice grains. AtIRT1 is therefore a valuable transporter for iron biofortification programs when used in combination with other genes encoding iron transporters and/or storage proteins. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

NOD promoter-controlled AtIRT1 expression functions synergistically with NAS and FERRITIN genes to increase iron in rice grains

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Publisher
Springer Netherlands
Copyright
Copyright © 2015 by The Author(s)
Subject
Life Sciences; Plant Sciences; Biochemistry, general; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1007/s11103-015-0404-0
Publisher site
See Article on Publisher Site

Abstract

Rice is a staple food for over half of the world’s population, but it contains only low amounts of bioavailable micronutrients for human nutrition. Consequently, micronutrient deficiency is a widespread health problem among people who depend primarily on rice as their staple food. Iron deficiency anemia is one of the most serious forms of malnutrition. Biofortification of rice grains for increased iron content is an effective strategy to reduce iron deficiency. Unlike other grass species, rice takes up iron as Fe(II) via the IRON REGULATED TRANSPORTER (IRT) in addition to Fe(III)-phytosiderophore chelates. We expressed Arabidopsis IRT1 (AtIRT1) under control of the Medicago sativa EARLY NODULIN 12B promoter in our previously developed high-iron NFP rice lines expressing NICOTIANAMINE SYNTHASE (AtNAS1) and FERRITIN. Transgenic rice lines expressing AtIRT1 alone had significant increases in iron and combined with NAS and FERRITIN increased iron to 9.6 µg/g DW in the polished grains that is 2.2-fold higher as compared to NFP lines. The grains of AtIRT1 lines also accumulated more copper and zinc but not manganese. Our results demonstrate that the concerted expression of AtIRT1, AtNAS1 and PvFERRITIN synergistically increases iron in both polished and unpolished rice grains. AtIRT1 is therefore a valuable transporter for iron biofortification programs when used in combination with other genes encoding iron transporters and/or storage proteins.

Journal

Plant Molecular BiologySpringer Journals

Published: Nov 11, 2015

References

  • Alfalfa Enod12 genes are differentially regulated during nodule development by Nod factors and Rhizobium invasion
    Bauer, P; Crespi, MD; Szecsi, J
  • Mutation in nicotianamine aminotransferase stimulated the Fe(II) acquisition system and led to iron accumulation in rice
    Cheng, L; Wang, F; Shou, H
  • The ZIP family of metal transporters
    Guerinot, ML
  • Rice plants take up iron as an Fe3+-phytosiderophore and as Fe2+
    Ishimaru, Y; Suzuki, M; Tsukamoto, T
  • Rice metal-nicotianamine transporter, OsYSL2, is required for the long-distance transport of iron and manganese
    Ishimaru, Y; Masuda, H; Bashir, K
  • Iron uptake, translocation, and regulation in higher plants
    Kobayashi, T; Nishizawa, NK
  • Over-expression of OsIRT1 leads to increased iron and zinc accumulations in rice
    Lee, S; An, G
  • Disruption of OsYSL15 leads to iron inefficiency in rice plants
    Lee, S; Chiecko, JC; Kim, SA
  • Genetic engineering approaches to improve the bioavailability and the level of iron in rice grains
    Lucca, P; Hurrell, R; Potrykus, I

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