1070-4272/02/7505-0829$27.00C2002 MAIK [Nauka/Interperiodica]
Russian Journal of Applied Chemistry, Vol. 75, No. 5, 2002, pp. 829!833. Translated from Zhurnal Prikladnoi Khimii, Vol. 75, No. 5,
2002, pp. 847!851.
Original Russian Text Copyright + 2002 by Tikhova, Shakirov, Fadeeva, Dergacheva, Samutenko, Fedorova.
AND POLYMERIC MATERIALS
NMR Study of Structural Changes in Humic Acids
of Meadow Soddy Soils
V. D. Tikhova, M. M. Shakirov, V. P. Fadeeva, M. I. Dergacheva,
L. V. Samutenko, and L. V. Fedorova
Institute of Organic Chemistry, Siberian Division, Russian Academy of Sciences, Novosibirsk, Russia
Institute of Soil Science and Agricultural Chemistry, Siberian Division, Russian Academy of Sciences,
Sakhalin Agriculture Research Institute, Far Eastern Scientific Center, Russian Agricultural Academy,
Yuzhnosakhalinsk, Novoaleksandrovskoe, Russia
Received June 21, 2001; in final form, January 2002
Abstract-Quantitative and qualitative changes in humic acids of meadow soddy soils, occurring under
the action of various fertilizer systems, were studied by
NMR spectroscopy is an extremely promising
method of studying the structure of soil organic sub-
stance whose main components are humic acids
(HAs). Since the late 1970s, various soils were ex-
tensively studied by
H NMR . In 1980,
P NMR was first used for studying alkaline soils
extracts . Only this method allowed identification
of various forms of phosphorus addition to HA macro-
molecule and estimation of quantitative relationships
between these species. Therefore, this method is wide-
ly used for studying various types of soils and soil
fractions . Data on transformations of soil
organic components under the action of cultivation are
Here we report on the quantitative and qualitative
changes proceeding in HAs of meadow soddy soils
under the action of various fertilizer systems, as
We studied the HAs of soils subjected to the action
of fertilizers under the stationary field conditions.
Peat3manure compost (C) was used as an organic
The experiments were carried out at the Novoaleksandrovskoe
pilot station (Sakhalin Island) since 1990. The soil of the pilot
parcel was meadow soddy, medium-loamy, and old arable. At
the pilot plots, feed crop rotation was maintained for 8 years.
The fertilizers were added three times at 1-year intervals and
then, after three years (in 1994). In all runs, the soil samples
were withdrawn from a depth of 0320 cm.
fertilizer. A mixture of granulated ammonium ni-
, granulated double superphosphate
, and potassium chloride KCl were used
as mineral fertilizers. The amount and composition of
the added fertilizers are listed in Table 1. Humic acids
were recovered by the standard procedure .
The total phosphorus content was determined spec-
trophotometrically with the vanadium3molybdenum
reagent after combustion of the samples in a flask
filled with oxygen.
To obtain NMR spectra, 50 mg of an HA sample
was dissolved in 0.5 ml of a 0.5 M NaOH solution
O and placed into an ampule of 5 mm outer
Table 1. Amount and composition of the added fertilizers
³ Amount of added fertilizers,
ple no. ³³kg ha
1 ³Control (no ³ !
2 ³1NPK ³N60P90K90*
3 ³1C ³100 10
4 ³2C ³200 10
5 ³1NPK + 2C ³N60P90K90 + 20010
6 ³3NPK + 1C ³N180P270K270 + 100 10
7 ³3NPK + 2C ³N180P270K270 + 200 10
* (NPK) Active elements of a mineral fertilizer (nitrogen, phos-
phorus, and potassium); the number to the right of the corre-
sponding element corresponds to the amount of this element
in kilograms added per hectare of the soil, evaluated from
the formulas of components of the mixed fertilizer.