Arch Virol (1997) 142: 1881±1887
Nigerian rotavirus serotype G8 could not be typed
by PCR due to nucleotide mutation at the 3
of the primer binding site
M. I. Adah
, A. Rohwedder
, O. D. Olaleyle
, and H. Werchau
Department of Medical Microbiology and Virology,
t, Bochum, Germany
Department of Virology, University College Hospital,
College of Medicine, University of Ibadan, Nigeria
Accepted April 21, 1997
Summary. A rotavirus strain HMG89 from Nigeria with short electrophoretic
pattern was typed G3 by PCR. A cDNA clone from the PCR product which
hybridised in Northern blots to RNA segment 9 of the homologous Nigerian
rotavirus strain HMG89 and laboratory reference strain 69M but not to other
mammalian group A rotaviruses was sequenced. The VP7 gene 9 sequence is
1060 nucleotides long with two base deletions at positions 1034±1035.
Sequence analysis of the primer (aAT8) used in the previous PCR serotyping
assay revealed a mutation in one of the three nucleotide bases at the 3
the primer binding site accounting for our inability to serotype G8 strains in our
samples. These ®ndings demonstrate that PCR analysis can, albeit infrequently,
lead to error in typing of rotaviruses due to small numbers of mutations in the
primer binding region.
Rotaviruses are the single most important etiologic agents of severe diarrheal
illness of infants and young children throughout the world  and major cause
of childhood death in the less developed countries .
The outer shell of the rotavirus capsid consists of two independent
neutralisation antigens, VP7 and VP4, both of which elicit neutralisation and
Daad research fellow on study leave from the Department of Veterinary Medicine,
University of Maiduguri, Nigeria.