Nicotiana benthamiana plants expressing beet necrotic yellowvein virus (BNYVV) coat protein-specific scFv are partiallyprotected against the establishment of the virus inthe early stages of infection and its pathogenic effectsin the late stages of infection

Nicotiana benthamiana plants expressing beet necrotic yellowvein virus (BNYVV) coat... Transgenic plants of Nicotiana benthamiana expressing single chain antibody fragments (scFv) specific for the coat protein of beet necrotic yellow vein virus (BNYVV) and non-expressing control plants were inoculated with BNYVV mechanically and by means of the vector Polymyxa betae . The scFv were presumably expressed in the endoplasmic reticulum (ER). The average time needed for infections to become detectable was longer in the scFv-expressing plants than in the non-expressing control plants. In addition, the scFv-expressing plants were partially protected against the pathogenic effects exerted by the virus on N. benthamiana plants in the late stages of infection. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Nicotiana benthamiana plants expressing beet necrotic yellowvein virus (BNYVV) coat protein-specific scFv are partiallyprotected against the establishment of the virus inthe early stages of infection and its pathogenic effectsin the late stages of infection

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Publisher
Springer-Verlag
Copyright
Copyright © Wien by 1997 Springer-Verlag/
Subject
Legacy
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s007050050203
Publisher site
See Article on Publisher Site

Abstract

Transgenic plants of Nicotiana benthamiana expressing single chain antibody fragments (scFv) specific for the coat protein of beet necrotic yellow vein virus (BNYVV) and non-expressing control plants were inoculated with BNYVV mechanically and by means of the vector Polymyxa betae . The scFv were presumably expressed in the endoplasmic reticulum (ER). The average time needed for infections to become detectable was longer in the scFv-expressing plants than in the non-expressing control plants. In addition, the scFv-expressing plants were partially protected against the pathogenic effects exerted by the virus on N. benthamiana plants in the late stages of infection.

Journal

Archives of VirologySpringer Journals

Published: Sep 1, 1997

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