Nhumirim virus, a novel flavivirus isolated from mosquitoes from the Pantanal, Brazil

Nhumirim virus, a novel flavivirus isolated from mosquitoes from the Pantanal, Brazil We describe the isolation of a novel flavivirus, isolated from a pool of mosquitoes identified as Culex ( Culex ) chidesteri collected in 2010 in the Pantanal region of west-central Brazil. The virus is herein designated Nhumirim virus (NHUV) after the name of the ranch from which the mosquito pool was collected. Flavivirus RNA was detected by real-time RT-PCR of homogenized mosquitoes and from the corresponding C6/36 culture supernatant. Based on full-genome sequencing, the virus isolate was genetically distinct from but most closely related to Barkedji virus (BJV), a newly described flavivirus from Senegal. Phylogenetic analysis demonstrated that NHUV grouped with mosquito-borne flaviviruses forming a clade with BJV. This clade may be genetically intermediate between the Culex -borne flaviviruses amplified by birds and the insect-only flaviviruses. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Nhumirim virus, a novel flavivirus isolated from mosquitoes from the Pantanal, Brazil

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Publisher
Springer Journals
Copyright
Copyright © 2015 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-014-2219-8
Publisher site
See Article on Publisher Site

Abstract

We describe the isolation of a novel flavivirus, isolated from a pool of mosquitoes identified as Culex ( Culex ) chidesteri collected in 2010 in the Pantanal region of west-central Brazil. The virus is herein designated Nhumirim virus (NHUV) after the name of the ranch from which the mosquito pool was collected. Flavivirus RNA was detected by real-time RT-PCR of homogenized mosquitoes and from the corresponding C6/36 culture supernatant. Based on full-genome sequencing, the virus isolate was genetically distinct from but most closely related to Barkedji virus (BJV), a newly described flavivirus from Senegal. Phylogenetic analysis demonstrated that NHUV grouped with mosquito-borne flaviviruses forming a clade with BJV. This clade may be genetically intermediate between the Culex -borne flaviviruses amplified by birds and the insect-only flaviviruses.

Journal

Archives of VirologySpringer Journals

Published: Jan 1, 2015

References

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