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Z. Sváb, P. Hajdukiewicz, P. Maliga (1990)
Stable transformation of plastids in higher plants.Proceedings of the National Academy of Sciences of the United States of America, 87
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M. Yukawa, T. Tsudzuki, M. Sugiura (2006)
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J. Staub, P. Maliga (1995)
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H. Koop, S. Herz, T. Golds, J. Nickelsen (2007)
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P. Maliga (2004)
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P. Maliga (2003)
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J. Staub, P. Maliga (1993)
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H. Carrer, J. Staub, P. Maliga (1991)
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J. Palmer (1985)
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S. Chakrabarti, K. Lutz, Benjawan Lertwiriyawong, Z. Sváb, P. Maliga (2006)
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C. Lelivelt, M. McCabe, C. Newell, C. deSnoo, Kees Dun, I. Birch-Machin, J. Gray, K. Mills, J. Nugent (2005)
Stable Plastid Transformation in Lettuce (Lactuca sativa L.)Plant Molecular Biology, 58
Z. Sváb, P. Maliga (1993)
High-frequency plastid transformation in tobacco by selection for a chimeric aadA gene.Proceedings of the National Academy of Sciences of the United States of America, 90
Tarinee Tungsuchat, H. Kuroda, J. Narangajavana, P. Maliga (2006)
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S. Ruf, M. Hermann, I. Berger, H. Carrer, R. Bock (2001)
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D. Verma, H. Daniell (2007)
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H. Carrer, P. Maliga (1995)
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K. Lutz, Z. Sváb, P. Maliga (2006)
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K. Wostrikoff, D. Stern (2007)
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W. Stiekema, F. Heidekamp, W. Dirkse, J. Beckum, P. Haan, Carolien Bosch, J. Louwerse (1988)
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M. Sugiura (1992)
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HU Koop, S Herz, TJ Golds, J Nickelsen (2007)
Cell and molecular biology of plastids (topics in current genetics)
Fei Zhou, Daniel Karcher, R. Bock (2007)
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H. Daniell, S. Chebolu, S. Kumar, Michael Singleton, R. Falconer (2005)
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R. Bock (2007)
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Nathalie Dufourmantel, B. Pélissier, Frédéric Garçon, G. Peltier, J. Ferullo, G. Tissot (2004)
Generation of fertile transplastomic soybeanPlant Molecular Biology, 55
R. Bock, H. Kossel, P. Maliga (1994)
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H. Carrer, Tish Hockenberry, Z. Sváb, P. Maliga (1993)
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Plastid transformation vectors are E. coli plasmids carrying a plastid marker gene for selection, adjacent cloning sites and flanking plastid DNA to target insertions in the plastid genome by homologous recombination. We report here on a family of next generation plastid vectors carrying synthetic DNA vector arms targeting insertions in the rbcL-accD intergenic region of the tobacco (Nicotiana tabacum) plastid genome. The pSS22 plasmid carries only synthetic vector arms from which the undesirable restriction sites have been removed by point mutations. The pSS24 vector carries a c-Myc tagged spectinomycin resistance (aadA) marker gene whereas in vector pSS30 aadA is flanked with loxP sequences for post-transformation marker excision. The synthetic vectors will enable direct manipulation of passenger genes in the transformation vector targeting insertions in the rbcL-accD intergenic region that contains many commonly used restriction sites.
Plant Molecular Biology – Springer Journals
Published: Apr 23, 2009
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