This study was designed to determine the role of a new temperate DNA phage BcP15 in relation to drug resistance. The multidrug resistant Shigella flexneri NK1925 was isolated from a patient of Infectious Diseases Hospital, Kolkata, India. This strain contained five plasmids ranging in size from 3 to 212 kb. After curing of five plasmids, this strain became sensitive to antibiotics. A plasmidless multidrug-resistant strain Burkholderia cepacia DR11 was isolated during the survey of microorganisms from coastal waters of deltaic Sunderbans. This strain always released a temperate phage BcP15 into culture supernatant. Turbid plaque formation was observed on the lawn of a plasmidless version (Pl − 35) of Shigella flexneri NK1925. A few distinct clones (Pl − 35R) appeared within the region of each plaque after 18 h incubation. S. flexneri NK1925, Pl − 35, and Pl − 35R clones showed the same PFGE band pattern of Xba I-digested chromosomal DNA. However, Pl − 35R clones were resistant to co-trimoxazole, trimethoprim, and eryth- romycin, to which B. cepacia DR11 was also resistant. Southern hybridization results indicated that these three antibiotic resistances in Pl − 35R clones were due to a BcP15 phage lysogen in the Pl − 35 version of S. flexneri NK1925.
Archives of Virology – Springer Journals
Published: Jul 1, 2006
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