New Mutations of SRMGenes in Saccharomyces cerevisiae and Certain Characteristics of Their Phenotypic Effects

New Mutations of SRMGenes in Saccharomyces cerevisiae and Certain Characteristics of Their... The effects of the previously identified mutations in nuclear genes SRM8, SRM12, SRM15, and SRM17on the maintenance of chromosomes and recombinant plasmids in Saccharomyces cerevisiaecells and on cell sensitivity to ionizing radiation were studied. The srm8mutation caused an increase in spontaneous chromosome loss in diploid cells. In yeast cells with the intact mitochondrial genome, all examined srmmutations decreased the mitotic stability of a centromeric recombinant plasmid with the chromosomal ARS element. Mutations srm12, srm15, and srm17also decreased the mitotic stability of a centromereless plasmid containing the same ARS element, whereas the srm8mutation did not markedly affect the maintenance of this plasmid. Mutations srm8, srm12, and srm17were shown to increase cell sensitivity to γ-rays. The SRM8gene was mapped, cloned, and found to correspond to the open reading frame YJLO76w in chromosome X. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Genetics Springer Journals

New Mutations of SRMGenes in Saccharomyces cerevisiae and Certain Characteristics of Their Phenotypic Effects

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Publisher
Springer Journals
Copyright
Copyright © 2001 by MAIK “Nauka/Interperiodica”
Subject
Biomedicine; Human Genetics
ISSN
1022-7954
eISSN
1608-3369
D.O.I.
10.1023/A:1011905429732
Publisher site
See Article on Publisher Site

Abstract

The effects of the previously identified mutations in nuclear genes SRM8, SRM12, SRM15, and SRM17on the maintenance of chromosomes and recombinant plasmids in Saccharomyces cerevisiaecells and on cell sensitivity to ionizing radiation were studied. The srm8mutation caused an increase in spontaneous chromosome loss in diploid cells. In yeast cells with the intact mitochondrial genome, all examined srmmutations decreased the mitotic stability of a centromeric recombinant plasmid with the chromosomal ARS element. Mutations srm12, srm15, and srm17also decreased the mitotic stability of a centromereless plasmid containing the same ARS element, whereas the srm8mutation did not markedly affect the maintenance of this plasmid. Mutations srm8, srm12, and srm17were shown to increase cell sensitivity to γ-rays. The SRM8gene was mapped, cloned, and found to correspond to the open reading frame YJLO76w in chromosome X.

Journal

Russian Journal of GeneticsSpringer Journals

Published: Oct 16, 2004

References

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