New factors regulating magnesium chelatase in the green alga Chlamydomonas reinhardtii

New factors regulating magnesium chelatase in the green alga Chlamydomonas reinhardtii The unicellular green alga Chamydomonas reinhardtii, as distinct from higher plants synthesizing chlorophyll (Chl) only in the light, produces it also in darkness. The mechanisms of dark Chl biosynthesis were studied by biochemical and genetic methods on C. reinhardtii pigment LTS3 mutants, their revertants, and reverse mutants; LTS3 gene encodes a transcription factor regulating the genes of the key enzyme of Chl biosynthesis, magnesium chelatase (MgCh). The cells of LTS3 mutants accumulate in darkness the MgCh substrate, protoporphyrin IX, and produce orange colonies greening in the light. It turned out that the phenotype of these mutants is determined by a decrease of MgCh activity under conditions of heterotrophic growth. In the study of lts3 mutation suppression, two new nuclear genes of C. reinhardtii, SUP-3 and SUP-1, encoding factors regulating MgCh activity were found. SUP-3 is linked with LTS3 and encodes a protein repressor of alternative to LTS3 pathway of MgCh regulation. Molecular identification of the SUP-1 gene allows a suggestion that its product is a component of the LTS3 pathway, controls the process of greening, and regulates MgCh at the posttranslational level. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

New factors regulating magnesium chelatase in the green alga Chlamydomonas reinhardtii

Loading next page...
 
/lp/springer_journal/new-factors-regulating-magnesium-chelatase-in-the-green-alga-10GhQPlny3
Publisher
Springer US
Copyright
Copyright © 2014 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Plant Physiology; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443714020034
Publisher site
See Article on Publisher Site

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches

$49/month

Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.

$588

$360/year

billed annually
Start Free Trial

14-day Free Trial