Nerve regeneration after local administration of the inhibitor peptide of the TGF-ß1 (P144®)

Nerve regeneration after local administration of the inhibitor peptide of the TGF-ß1 (P144®) To determine whether local administration of the inhibitor peptide of the transforming growth factor-β1 (TGF-β1; p144®) at the site of the anastomosis of a peripheral nerve can improve the peripheral nerve regeneration. Sciatic nerves were transected and immediately repaired. Wistar rats ( n = 45) were divided into three groups: a control group (group I, n = 15), a vehicle solution group (group II, n = 15), and a p144® - treated group (group III, n = 15); 0.25 ml of a solution containing 2.5 mg of p144® per milliliter of tissucol® was administered at the site of the nerve repair in group III. The animals were sacrificed at 12 weeks. Spinal motor neurons and distal axons were quantified. Electrophysiological studies and walking track analysis (WTA) were performed at the end of the survival period. Immunohistological study for TGF-ß1 around the nerve anastomosis was performed. The statistical comparisons between the mean number of neurons ( p = 0.27) and distal axons ( p = 0.32) in group I, II, and III showed no statistical differences between groups. However, the amplitude of the electrophysiological studies showed a statistically significant difference between the p144®-treated group III and the control group at 2 months postoperatively ( p = 0.016). Moreover, WTA showed no statistically significant differences between groups ( p > 0.1) except in the p144® group, which was higher than control group I at 2 months postoperatively ( p = 0.019). A significant decrease in global cellularity ( p = 0.016) and immunohistological activity for TGF-ß1 ( p = 0.012) around the nerve anastomosis was found in the group of animals treated with the inhibitor peptide of TGF-β1. P144® delivered at the site of nerve anastomosis demonstrates a capacity to improve the amplitude of electrophysiological studies and WTA at 2 months postoperatively, which might ensure acceleration of the peripheral nerve regeneration by diminishing the scar tissue around the nerve anastomosis. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png European Journal of Plastic Surgery Springer Journals

Nerve regeneration after local administration of the inhibitor peptide of the TGF-ß1 (P144®)

Loading next page...
 
/lp/springer_journal/nerve-regeneration-after-local-administration-of-the-inhibitor-peptide-Y0migJASDX
Publisher
Springer-Verlag
Copyright
Copyright © 2010 by Springer-Verlag
Subject
Medicine & Public Health; Plastic Surgery
ISSN
0930-343X
eISSN
1435-0130
D.O.I.
10.1007/s00238-010-0406-8
Publisher site
See Article on Publisher Site

Abstract

To determine whether local administration of the inhibitor peptide of the transforming growth factor-β1 (TGF-β1; p144®) at the site of the anastomosis of a peripheral nerve can improve the peripheral nerve regeneration. Sciatic nerves were transected and immediately repaired. Wistar rats ( n = 45) were divided into three groups: a control group (group I, n = 15), a vehicle solution group (group II, n = 15), and a p144® - treated group (group III, n = 15); 0.25 ml of a solution containing 2.5 mg of p144® per milliliter of tissucol® was administered at the site of the nerve repair in group III. The animals were sacrificed at 12 weeks. Spinal motor neurons and distal axons were quantified. Electrophysiological studies and walking track analysis (WTA) were performed at the end of the survival period. Immunohistological study for TGF-ß1 around the nerve anastomosis was performed. The statistical comparisons between the mean number of neurons ( p = 0.27) and distal axons ( p = 0.32) in group I, II, and III showed no statistical differences between groups. However, the amplitude of the electrophysiological studies showed a statistically significant difference between the p144®-treated group III and the control group at 2 months postoperatively ( p = 0.016). Moreover, WTA showed no statistically significant differences between groups ( p > 0.1) except in the p144® group, which was higher than control group I at 2 months postoperatively ( p = 0.019). A significant decrease in global cellularity ( p = 0.016) and immunohistological activity for TGF-ß1 ( p = 0.012) around the nerve anastomosis was found in the group of animals treated with the inhibitor peptide of TGF-β1. P144® delivered at the site of nerve anastomosis demonstrates a capacity to improve the amplitude of electrophysiological studies and WTA at 2 months postoperatively, which might ensure acceleration of the peripheral nerve regeneration by diminishing the scar tissue around the nerve anastomosis.

Journal

European Journal of Plastic SurgerySpringer Journals

Published: Jun 1, 2010

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve Freelancer

DeepDyve Pro

Price
FREE
$49/month

$360/year
Save searches from
Google Scholar,
PubMed
Create lists to
organize your research
Export lists, citations
Read DeepDyve articles
Abstract access only
Unlimited access to over
18 million full-text articles
Print
20 pages/month
PDF Discount
20% off