NE-11 represents a new strain variant class of Potato virus Y

NE-11 represents a new strain variant class of Potato virus Y This report describes the characterization by whole-genome sequencing of four PVY isolates with unique combinations of molecular and symptomatic characteristics. Three of these four isolates were of type PVY N:O (ID-1, OR-1, PN10A), including one of “type B”, which contains an extra recombination event in the 5′UTR/P1 cistron; the other (NE-11) represents a novel PVY molecular genotype, previously misclassified as a PVY NA-NTN isolate. The full genome sequence of this latter isolate is unique inasmuch as it is nearly identical to that of PVY N isolates for the first 2,000 nucleotides (nts), after which it very strongly resembles PVY NA-NTN isolates for the next 600 nts. For the final 7,000 nts of its genome, NE-11 shares intermediate identity with these other two previously reported classes of PVY N genomes, except for a portion of the capsid protein region in which it resembles neither. Recombination in each of the four isolates was verified by a suite of recombination detection programs. PN10A represents the first complete sequence of a PVY strain variant of the class reported as PVY N -W (or PVY N:O ) type B. Specific PCR assays for two unique regions of NE-11 are presented that will allow the identification of this strain variant by other researchers. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

NE-11 represents a new strain variant class of Potato virus Y

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Publisher
Springer Journals
Copyright
Copyright © 2008 by Springer-Verlag
Subject
Biomedicine; Infectious Diseases; Medical Microbiology ; Virology
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-007-0030-5
Publisher site
See Article on Publisher Site

Abstract

This report describes the characterization by whole-genome sequencing of four PVY isolates with unique combinations of molecular and symptomatic characteristics. Three of these four isolates were of type PVY N:O (ID-1, OR-1, PN10A), including one of “type B”, which contains an extra recombination event in the 5′UTR/P1 cistron; the other (NE-11) represents a novel PVY molecular genotype, previously misclassified as a PVY NA-NTN isolate. The full genome sequence of this latter isolate is unique inasmuch as it is nearly identical to that of PVY N isolates for the first 2,000 nucleotides (nts), after which it very strongly resembles PVY NA-NTN isolates for the next 600 nts. For the final 7,000 nts of its genome, NE-11 shares intermediate identity with these other two previously reported classes of PVY N genomes, except for a portion of the capsid protein region in which it resembles neither. Recombination in each of the four isolates was verified by a suite of recombination detection programs. PN10A represents the first complete sequence of a PVY strain variant of the class reported as PVY N -W (or PVY N:O ) type B. Specific PCR assays for two unique regions of NE-11 are presented that will allow the identification of this strain variant by other researchers.

Journal

Archives of VirologySpringer Journals

Published: Mar 1, 2008

References

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