+ + Key message Suppression of AtVHA-c5 expression results in changes in H and Na fluxes of roots, and increase sensitivity to salt in Arabidopsis. Abstract Vacuolar-type H -ATPase (V-ATPase), a multisubunit endomembrane proton pump, is essential in plant growth and response to environmental stresses. In the present study, the function of Arabidopsis V-ATPase subunit c5 (AtVHA-c5) gene in response to salt stress was investigated. Subcellular localization showed that AtVHA-c5 was mainly localized to endosomes and the vacuolar membrane in Arabidopsis. The analysis of quantitative real-time PCR showed that expression of AtVHA-c5 gene was induced by NaCl stress. Histochemical analysis revealed that AtVHA-c5 was expressed in the root epidermis of untreated Arabidopsis and in the whole root elongation zone after NaCl treatment. Phenotypic analysis showed that the atvha-c5 mutant is sensitive to high NaCl as compared to the wild type. The non-invasive micro-test technology + + measurement demonstrated that the net H and Na efflux in the root elongation zone of the atvha-c5 mutant was weaker + + than that of the wild type under NaCl treatment, suggesting that H and N a fluxes in atvha-c5 roots are impaired under NaCl stress. Moreover, compared to the wild type, the
Plant Cell Reports – Springer Journals
Published: Jan 6, 2018
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