NaCl-induced expression of AtVHA-c5 gene in the roots plays a role in response of Arabidopsis to salt stress

NaCl-induced expression of AtVHA-c5 gene in the roots plays a role in response of Arabidopsis to... + + Key message Suppression of AtVHA-c5 expression results in changes in H and Na fluxes of roots, and increase sensitivity to salt in Arabidopsis. Abstract Vacuolar-type H -ATPase (V-ATPase), a multisubunit endomembrane proton pump, is essential in plant growth and response to environmental stresses. In the present study, the function of Arabidopsis V-ATPase subunit c5 (AtVHA-c5) gene in response to salt stress was investigated. Subcellular localization showed that AtVHA-c5 was mainly localized to endosomes and the vacuolar membrane in Arabidopsis. The analysis of quantitative real-time PCR showed that expression of AtVHA-c5 gene was induced by NaCl stress. Histochemical analysis revealed that AtVHA-c5 was expressed in the root epidermis of untreated Arabidopsis and in the whole root elongation zone after NaCl treatment. Phenotypic analysis showed that the atvha-c5 mutant is sensitive to high NaCl as compared to the wild type. The non-invasive micro-test technology + + measurement demonstrated that the net H and Na efflux in the root elongation zone of the atvha-c5 mutant was weaker + + than that of the wild type under NaCl treatment, suggesting that H and N a fluxes in atvha-c5 roots are impaired under NaCl stress. Moreover, compared to the wild type, the http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Cell Reports Springer Journals

NaCl-induced expression of AtVHA-c5 gene in the roots plays a role in response of Arabidopsis to salt stress

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Publisher
Springer Berlin Heidelberg
Copyright
Copyright © 2018 by Springer-Verlag GmbH Germany, part of Springer Nature
Subject
Life Sciences; Plant Sciences; Cell Biology; Biotechnology; Plant Biochemistry
ISSN
0721-7714
eISSN
1432-203X
D.O.I.
10.1007/s00299-017-2241-x
Publisher site
See Article on Publisher Site

Abstract

+ + Key message Suppression of AtVHA-c5 expression results in changes in H and Na fluxes of roots, and increase sensitivity to salt in Arabidopsis. Abstract Vacuolar-type H -ATPase (V-ATPase), a multisubunit endomembrane proton pump, is essential in plant growth and response to environmental stresses. In the present study, the function of Arabidopsis V-ATPase subunit c5 (AtVHA-c5) gene in response to salt stress was investigated. Subcellular localization showed that AtVHA-c5 was mainly localized to endosomes and the vacuolar membrane in Arabidopsis. The analysis of quantitative real-time PCR showed that expression of AtVHA-c5 gene was induced by NaCl stress. Histochemical analysis revealed that AtVHA-c5 was expressed in the root epidermis of untreated Arabidopsis and in the whole root elongation zone after NaCl treatment. Phenotypic analysis showed that the atvha-c5 mutant is sensitive to high NaCl as compared to the wild type. The non-invasive micro-test technology + + measurement demonstrated that the net H and Na efflux in the root elongation zone of the atvha-c5 mutant was weaker + + than that of the wild type under NaCl treatment, suggesting that H and N a fluxes in atvha-c5 roots are impaired under NaCl stress. Moreover, compared to the wild type, the

Journal

Plant Cell ReportsSpringer Journals

Published: Jan 6, 2018

References

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