RNA polymerase (RNAP) exhibits absolute processivity being capable of synthesizing RNA 103–105 nucleotides in length without breaking contact with the DNA template. Stability of the elongation complex is thought to depend, in particular, on the RNAP–DNA interactions downstream along the run of transcription. We studied the effects of several deletions and insertions in the RNAP β"-subunit N-terminal region, which presumably interacts with the downstream DNA duplex in the elongation complex. Most of the mutations obtained led to gross defects in RNAP assembly and disturbed catalytic activity of the enzyme. The mutations reduced stability of both promoter and elongation complexes, probably because they altered the contacts between RNAP and the downstream DNA duplex.
Russian Journal of Genetics – Springer Journals
Published: Oct 13, 2004
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