Mutations altering the specificity of the sensor RNA encoded by the Bacillus subtilis pbuE gene

Mutations altering the specificity of the sensor RNA encoded by the Bacillus subtilis pbuE gene Site-directed mutagenesis was used to study the leader region of the Bacillus subtilis pbuE gene, which codes for adenine-specific sensor RNA. Two nucleotide substitutions, 70U → C and A100 → G, in the conserved region (A box) of the leader changed the specificity of the sensor RNA in vivo: guanine acted as a positive transcription effector in place of adenine. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Genetics Springer Journals

Mutations altering the specificity of the sensor RNA encoded by the Bacillus subtilis pbuE gene

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Publisher
Nauka/Interperiodica
Copyright
Copyright © 2007 by Pleiades Publishing, Inc.
Subject
Biomedicine; Human Genetics; Animal Genetics and Genomics; Microbial Genetics and Genomics
ISSN
1022-7954
eISSN
1608-3369
D.O.I.
10.1134/S1022795407060178
Publisher site
See Article on Publisher Site

Abstract

Site-directed mutagenesis was used to study the leader region of the Bacillus subtilis pbuE gene, which codes for adenine-specific sensor RNA. Two nucleotide substitutions, 70U → C and A100 → G, in the conserved region (A box) of the leader changed the specificity of the sensor RNA in vivo: guanine acted as a positive transcription effector in place of adenine.

Journal

Russian Journal of GeneticsSpringer Journals

Published: Jun 28, 2007

References

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