Site-directed mutagenesis was used to study the leader region of the Bacillus subtilis pbuE gene, which codes for adenine-specific sensor RNA. Two nucleotide substitutions, 70U → C and A100 → G, in the conserved region (A box) of the leader changed the specificity of the sensor RNA in vivo: guanine acted as a positive transcription effector in place of adenine.
Russian Journal of Genetics – Springer Journals
Published: Jun 28, 2007
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