Mutational analysis of the activator of late transcription, Alt, in the lactococcal bacteriophage TP901-1

Mutational analysis of the activator of late transcription, Alt, in the lactococcal bacteriophage... An activator protein, Alt, synthesized during the early state of lytic infection is required for transcription of the late operon in the lactococcal phage TP901-1. In order to identify amino acid residues in the Alt protein required for activation of the TP901-1 late promoter, P late , hydroxylamine mutagenesis was performed, resulting in almost saturating mutagenesis of alt . Twenty-three different non-functional alt alleles containing one, and in one case two amino acid exchanges were isolated and analyzed. Eight of the twenty-three mutant proteins were still able to activate the P late promoter to some extent. Our results show that alt encodes a protein of 16.7 kDa and that the last fourteen amino acids in the C-terminal part of the protein are required for activation of the P late promoter. By combining sequence analysis with experimental data we suggest that the C-terminal half of the Alt protein contains a helix-turn-helix-like motif involved in DNA binding. We also propose that the C-terminal half of the Alt protein may be involved in interactions with the bacterial RNA polymerase, whereas the N-terminal half of the protein is proposed to be important for the overall protein structure. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Mutational analysis of the activator of late transcription, Alt, in the lactococcal bacteriophage TP901-1

Loading next page...
 
/lp/springer_journal/mutational-analysis-of-the-activator-of-late-transcription-alt-in-the-kaiFcbC2Ka
Publisher
Springer-Verlag
Copyright
Copyright © 2007 by Springer-Verlag
Subject
Biomedicine; Medical Microbiology; Virology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-006-0851-7
Publisher site
See Article on Publisher Site

Abstract

An activator protein, Alt, synthesized during the early state of lytic infection is required for transcription of the late operon in the lactococcal phage TP901-1. In order to identify amino acid residues in the Alt protein required for activation of the TP901-1 late promoter, P late , hydroxylamine mutagenesis was performed, resulting in almost saturating mutagenesis of alt . Twenty-three different non-functional alt alleles containing one, and in one case two amino acid exchanges were isolated and analyzed. Eight of the twenty-three mutant proteins were still able to activate the P late promoter to some extent. Our results show that alt encodes a protein of 16.7 kDa and that the last fourteen amino acids in the C-terminal part of the protein are required for activation of the P late promoter. By combining sequence analysis with experimental data we suggest that the C-terminal half of the Alt protein contains a helix-turn-helix-like motif involved in DNA binding. We also propose that the C-terminal half of the Alt protein may be involved in interactions with the bacterial RNA polymerase, whereas the N-terminal half of the protein is proposed to be important for the overall protein structure.

Journal

Archives of VirologySpringer Journals

Published: Feb 1, 2007

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve Freelancer

DeepDyve Pro

Price
FREE
$49/month

$360/year
Save searches from
Google Scholar,
PubMed
Create lists to
organize your research
Export lists, citations
Read DeepDyve articles
Abstract access only
Unlimited access to over
18 million full-text articles
Print
20 pages/month
PDF Discount
20% off