Mutation in the Sp1 motif of the bovine leptin gene affects its expression

Mutation in the Sp1 motif of the bovine leptin gene affects its expression Leptin is expressed mainly by adipocytes and plays a crucial role in the regulation of energy expenditure, food intake, and adiposity. Using PCR-heteroduplex analysis and sequencing, we investigated a C/G substitution in the promoter region of the bovine leptin gene. Application of the electrophoretic mobility shift assay showed that the C→G transversion decreased the leptin gene promoter binding capacity for nuclear proteins. With real-time PCR and Western blotting, we showed that the leptin expression level was higher in cattle with the CC than with the GG genotype. Mammalian Genome Springer Journals

Mutation in the Sp1 motif of the bovine leptin gene affects its expression

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Copyright © 2006 by Springer Science+Business Media, Inc.
Life Sciences; Anatomy; Zoology; Cell Biology
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