The sodB gene encoding the only superoxide dismutase (Fe-SOD) in cells of the cyanobacterium Synechocystis sp. PCC 6803 was inactivated with gentamycin resistance aacC1 marker insertions located in the direct or reverse direction in the sodB gene. The corresponding ΔsodB12 and ΔsodB22 mutants are characterized by the complete absence of superoxide dismutase activity and the loss of viability upon standard photoautotrophic cultivation. Mutant cells can grow under conditions of a decreased illumination intensity and upon addition of NaHCO3 with catalase or bovine serum albumin in the growth medium. The ΔsodB22 mutant is auxotrophic for leucine due to the polar effect of insertion into the sodB gene on the downstream leuB gene controlling leucine biosynthesis. These data suggest that Fe-SOD is very important for providing resistance of Synechocystis 6803 cells to oxidative stress and thatsodB and leuB genes are organized into a single operon.
Russian Journal of Genetics – Springer Journals
Published: Oct 7, 2004
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