Multiple polyadenylated RNA viruses detected in pooled cultivated and wild plant samples

Multiple polyadenylated RNA viruses detected in pooled cultivated and wild plant samples RNA extracted from 120 leaf specimens from 17 plant species was pooled, and polyadenylated RNA species were sequenced together without barcoding in one lane using massively parallel sequencing technology. After analysis, complete or partial genome sequences representing 20 virus isolates of 16 polyadenylated RNA species were identified. In three cases, 2-3 distinct isolates of a virus species co-infected the same plant. Twelve of the viruses identified were described previously and belonged to the genera Potyvirus , Nepovirus, Allexivirus , and Carlavirus . Four were unknown and are proposed as members of the genera Potyvirus , Sadwavirus , and Trichovirus . Virus sequences were subsequently matched to original host plants using RT-PCR assays. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Multiple polyadenylated RNA viruses detected in pooled cultivated and wild plant samples

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Publisher
Springer Vienna
Copyright
Copyright © 2012 by Springer-Verlag
Subject
Biomedicine; Medical Microbiology; Virology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-011-1166-x
Publisher site
See Article on Publisher Site

Abstract

RNA extracted from 120 leaf specimens from 17 plant species was pooled, and polyadenylated RNA species were sequenced together without barcoding in one lane using massively parallel sequencing technology. After analysis, complete or partial genome sequences representing 20 virus isolates of 16 polyadenylated RNA species were identified. In three cases, 2-3 distinct isolates of a virus species co-infected the same plant. Twelve of the viruses identified were described previously and belonged to the genera Potyvirus , Nepovirus, Allexivirus , and Carlavirus . Four were unknown and are proposed as members of the genera Potyvirus , Sadwavirus , and Trichovirus . Virus sequences were subsequently matched to original host plants using RT-PCR assays.

Journal

Archives of VirologySpringer Journals

Published: Feb 1, 2012

References

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