Multi-center clinical evaluation of the Access AMH assay to determine AMH levels in reproductive age women during normal menstrual cycles

Multi-center clinical evaluation of the Access AMH assay to determine AMH levels in reproductive... Background AMH is widely used for assessing ovarian reserve, and it is particularly convenient, because it is thought to have minimal variability throughout the menstrual cycle. However, studies assessing the stability of AMH over the menstrual cycle have been conflicting. Purpose The purpose of this study is to determine whether AMH levels vary across the normal menstrual cycle. Design A multi-center, prospective cohort study conducted at three US centers. Methods Fifty females with regular menstrual cycles aged 18–45 underwent serial venipuncture every 3–5days startingin the early follicular phase and lasting up to 10 collections. AMH was tested using the Access 2 immunoassay system. Results Age-adjusted mixed-effect models utilizing data from 384 samples from 50 subjects demonstrated a within subject standard deviation of 0.81 (95% CI 0.75–0.88) with a coefficient of variation of 23.8% across the menstrual cycle and between subject standard deviation of 2.56 (95% CI 2.13–3.21) with a coefficient of variation of 75.1%. Intra-class correlation (ICC) of AMH across the menstrual cycle was 0.91. Conclusion Overall, AMH levels, using the automated Access AMH assay, appear to be relatively stable across the menstrual cycle. Fluctuations, if any, appear to be small, and therefore, clinicians may advise patients to have http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Assisted Reproduction and Genetics Springer Journals

Multi-center clinical evaluation of the Access AMH assay to determine AMH levels in reproductive age women during normal menstrual cycles

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Publisher
Springer US
Copyright
Copyright © 2018 by Springer Science+Business Media, LLC, part of Springer Nature
Subject
Medicine & Public Health; Gynecology; Reproductive Medicine; Human Genetics
ISSN
1058-0468
eISSN
1573-7330
D.O.I.
10.1007/s10815-018-1141-5
Publisher site
See Article on Publisher Site

Abstract

Background AMH is widely used for assessing ovarian reserve, and it is particularly convenient, because it is thought to have minimal variability throughout the menstrual cycle. However, studies assessing the stability of AMH over the menstrual cycle have been conflicting. Purpose The purpose of this study is to determine whether AMH levels vary across the normal menstrual cycle. Design A multi-center, prospective cohort study conducted at three US centers. Methods Fifty females with regular menstrual cycles aged 18–45 underwent serial venipuncture every 3–5days startingin the early follicular phase and lasting up to 10 collections. AMH was tested using the Access 2 immunoassay system. Results Age-adjusted mixed-effect models utilizing data from 384 samples from 50 subjects demonstrated a within subject standard deviation of 0.81 (95% CI 0.75–0.88) with a coefficient of variation of 23.8% across the menstrual cycle and between subject standard deviation of 2.56 (95% CI 2.13–3.21) with a coefficient of variation of 75.1%. Intra-class correlation (ICC) of AMH across the menstrual cycle was 0.91. Conclusion Overall, AMH levels, using the automated Access AMH assay, appear to be relatively stable across the menstrual cycle. Fluctuations, if any, appear to be small, and therefore, clinicians may advise patients to have

Journal

Journal of Assisted Reproduction and GeneticsSpringer Journals

Published: Mar 14, 2018

References

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