Mammalian Genome 10, 948 (1999). Incorporating Mouse Genome © Springer-Verlag New York Inc. 1999 1 2 Jeffrey D. Ceci, * Kathleen A. Mills ** Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, TX 77555-0643, USA Department of Pediatrics, University of Iowa, 206 MRc, Iowa City, IA 52242, USA Submitted: 14 January 1999 Introduction This latest chromosome (Chr) 8 report contains 995 entries, incor- suppressed recombination ~ 12 cM long around the Os locus seg- porating data available by 11/98. Of the mapped loci on this chro- regating in 2 crosses. The investigators suggested that an inversion mosome, 29% are genes/pseudogenes, 8% are expressed sequence may explain their data, but did not see one with G banding. tags (ESTs), and 63% are anonymous markers. There are 78 ad- Significant progress has been made this year toward establish- ditions to this year’s map, of which 42 are ESTs, 24 are genes, 3 ing a physical map of Chr 8. At present, 243 of the 359 Chr 8 are quantitative trait loci (QTLs), and 9 are anonymous markers. microsatellite loci from the Whitehead Institute/MIT Genome Re- The chromosome length of 84 cM is unchanged from the previous search Center have been placed on YAC clones. The microsatellite Chr 8 report. The most distal 12 cM of the chromosome has almost markers localized on the physical map are indicated with a “P” in no known loci, and none of the new entries fell into this region. the “methods” column of Table 1, and these data can be accessed The current consensus genetic map of Chr 8, a locus table (Table at http://carbon.wi.mit.edu:8000/cgi-bin/mouse/index. 1), and a cytogenetic map (Figure 1) can be accessed electronically Currently, 79 ESTs from 5 sources are localized to Chr 8. Of at The Jackson Laboratory World Wide Web site (http:// the 42 new entries, 41 are from the ERATO/Wayne State Univer- www.informatics.jax.org/bin/ccr/index). sity project (D8Ertd#). Most of the ESTs on Chr 8 have been mapped on the community resource of the BSS panel from The Jackson Laboratory. McCarthy et al. (J45215) published the first Consensus map construction mapping data for Chr 8 using the new mouse-hamster radiation hybrid panel. The method used to construct the map has been described in pre- vious Chr 8 reports. Briefly, a framework map was established using mapping data available from several interspecific back- Comparative mapping crosses. Then, loci from other crosses were placed on the map relative to their distances to markers used to establish the frame- Of the ~ 250 genes on Chr 8, ~ 50% have identified and mapped work map. Since the consensus map contains data from several human homologs. There are 17 genes with mapped rat homologs, different genetic crosses, readers are cautioned that there are likely and another 20 genes with rat homologs that have been identified to be many mistakes with regard to the gene order. Gene order but not yet localized. should not be inferred unless genes have been mapped relative to Proceeding from the centromere to the telomere on Chr 8 are each other in the same genetic cross. Readers are encouraged to genes whose homologs are found in the following 6 human chro- examine the primary mapping data from literature and from the mosomal locations: 19p13, 13q33-q34, 8p12-q21, 4q28-q35, Mouse Genome Database to determine if genes of interest have 19p13 (again), 16q12-qter and lq42-q43. There are several breaks been mapped relative to each other. in the regions of homology that could result from either inaccurate gene order or extensive evolutionary changes. The region of syn- teny with 16q is about 33 cM in length and is the largest region of Marker and mutation updates homology between Chr 8 and human chromosomes. For genes in the midregion of the chromosome (containing homologs to human Three new traits were reported this year (all QTLs), for a total of Chr 8, 4 and 19), mapping data are from many different crosses. 31 phenotypic variants mapped to Chr 8. The new traits are Char2 Consequently, it is difficult to determine gene order, or the pattern (malaria resistance), Orch6 (autoimmune orchitis resistance), and of homology with the human map. Grewal et al. (J48971) mapped Siafq1 (a female-specific response to stress induced analgesia). 12 genes, 8 with human Chr 4 homologs, in a single cross (the Seven of the 31 phenotypic loci on Chr 8 have been cloned. Prog- EUCIB BSS panel) as a start to clarifying these relationships. For ress was reported this year for fused toes (Ft ), nervous (nr ), and 2 other genes in mid-chromosome, the human homologs map out- oligosyndactylism (Os). Lesche et al. (J44450) identified a gene side known regions of synteny, and no corroborating mammalian deleted in Ft (a mutant generated by transgene insertion). This data is available to confirm or extend the homology. These are gene encodes a protein with similarities to ubiquitin-conjugating Plcd (3p22-p21.3), at ~ 21 cM, and Srebf1 (17p11.2), at ~ 33 cM. enzymes. De Jager et al. (J46798) reported evidence for a modifier Bartsch et al. (J47553) showed by FISH analysis that the homolog locus on Chr 5 for nr. Lenz et al. (J48984) observed a region of of Stk3 (~ 38 cM), previously thought to be on Chr 14, is actually on 19p. * Committee Chair Please send corrections/new information to Jeffrey Ceci ** Co-Chair (firstname.lastname@example.org) or Kathleen Mills (email@example.com. Correspondence to: J.D. Ceci uiowa.edu).
Mammalian Genome – Springer Journals
Published: Oct 1, 1999
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