Mammalian Genome 10, 947 (1999). Incorporating Mouse Genome © Springer-Verlag New York Inc. 1999 1 2 3 4 5 Robert W. Williams, * Joe M. Angel, ** Bernadette C. Holdener, Rebecca Oakey, Kent W. Hunter Center for Neuroscience, University of Tennessee, Memphis, 855 Monroe Avenue, Memphis, Tennessee 38163, USA Department of Carcinogenesis, University of Texas Science Park, Research Division, Smithville, Texas 78957, USA Department of Biochemistry and Cell Biology, SUNY at Stony Brook, Stony Brook, New York 11794-5215, USA Division of Genetics, Children’s Hospital of Philadelphia, 34th and Civic Center Boulevard, Philadelphia, Pennsylvania 19104, USA Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, Pennsylvania 19111, USA Submitted: 1 December 1998 Introduction A total of 1354 loci are listed in this year’s report, a 12% gain over Data from large multilocus crosses and recombinant inbred strains last year’s report. Half of these loci are genes or expressed se- were compiled and ordered (see Table 2). Relative positions of quence tags (ESTs), and collectively they represent approximately common loci were computed and these anchor loci were then used 20% of all genes on Chr 7. Most of this year’s new loci are ESTs to construct a framework map. Other genes and markers were (e.g., J:24194), a trend that will continue to gain momentum and integrated into the framework. The total sex-averaged genetic that will greatly facilitate cloning mutations and quantitative trait length of Chr 7 is 72–75 cM. Generating a useful consensus map loci. A set of 200 known genes and cDNAs that map to Chr 7 and for the proximal 20 cM of Chr 7 has not yet been possible. Many that have been partly or entirely sequenced is available at: Æ www. crosses have exploited M. spretus (SPRET/Ei). Unfortunately, ncbi.nlm.nih.gov/UniGene/æ . there are notable differences in chromosomal organization be- tween M. spretus and M. musculus. This is highlighted by Clc4-2, Mouse and human genomes a gene that maps ~ 4 cM on Chr 7 in domestic strains but to Chr X in M. spretus [J:26310, 27740]! Loci that appear to be confidently The fast pace of sequencing of the human genome provides yet mapped in one cross to proximal Chr 7 may map with different another impetus to refine and extend homologies between mouse order and position in other crosses. Physical maps of Chr 7 for chromsomes and their human counterparts. Thirty percent of genes C57BL/6J (the strain whose genome is expected to be sequenced) on mouse Chr 7 have known human homologs restricted to parts of should soon resolve part of this problem. chromosomes 10, 11, 15, 16, and 19 (see our Table 1 and Æ www. ncbi.nlm.nih.gov/Omim/Homology/mouse7.htmlæ ). Most notable is a very long segment of Chr 7 from the centromere to 23 cM that Physical maps corresponds to the majority of the long arm of human Chr 19 (see Æ www-bio.llnl.gov/bbrp/genome/html/chrom_map.htmlæ ). Over 180 genes and 400 ESTs have been mapped to human 19q and the Several yeast artificial chromosome (YAC) libraries with average great majority will have homologs that map to mouse Chr 7. insert sizes of 150–820 Kb have been constructed (J:1014, 43732, The short stretch of Chr 7 (~ 24 cM: Saa1 to Myod ) is the first 43240, 40403). The Whitehead library has been assembled into a of three regions that match 11p15. This is followed by a 16-cM- rough whole chromosome contig using microsatellite markers. long interval that corresponds to several pieces of the long arm of Bacterial artificial chromosome (BAC) and P1 artificial chromo- Chr 15 (q14, q21, and q26). A 6 cM interval from Tyr to Hbb some libraries (PAC) libraries are also now available commer- corresponds to human 11q13, and the next interval—from Hbb to cially or by contacting the original investigators directly (http:// Calca—correspond to a second stretch of 11p15. The distal 16 cM bacpac.med.buffalo.edu). The Whitehead/MIT Center has been de- of Chr 7 correspond in succession to parts of 16p12, 10q, 11q13, veloping a physical map for the mouse genome by screening the and 11p15. WI/MIT820 library with its MIT microsatellite loci. A significant proportion of the genome has been ordered into arrayed contigs Map construction (see www.genome.wi.mit.edu/cgi-bin/mouse/index). Most of Chr 7 has been assembled into doubly-linked contigs, anchored on the The committee’s map of Chr 7 is available on-line at Æ www. genetic map. Although this represents a valuable resource, inves- informatics.jax.org/bin/ccr/contents?&year41999& chr47æ . tigators should use this data with caution. BAC and PAC libraries represent the genomic organization more faithfully than YACs, * Committee Chair and for this reason, the YAC physical map is often used primarily ** Co-Chair as a source of clones and probes to develop BAC and PAC contigs Correspondence to: R.W. Williams across regions of interest.
Mammalian Genome – Springer Journals
Published: Oct 1, 1999
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