Mammalian Genome 10, 957 (1999). Incorporating Mouse Genome © Springer-Verlag New York Inc. 1999 1 2 Roger H. Reeves, * Deborah E. Cabin ** Department of Physiology, P203, Johns Hopkins School of Medicine, 725 N. Wolfe St., Baltimore, MD 21205 USA Laboratory of Genetic Disease Research, National Human Genome Research Institute, 49 Convent Dr., 49B67, MSC 4472, Bethesda, MD 20892-4472, USA Submitted: 1 December 1998 Introduction The eighth report for mouse Chromosome 16 (Chr 16) includes These are given a rating of 2. The lowest confidence rating of 3 is 706 markers, of which 102 are new this year. All but two of these given to markers localized only to a broad area by methods such as markers are localized on the chromosome, and several markers QTL analysis, in situ hybridization, somatic cell hybrid analysis, or listed as syntenic last year are localized on this year’s map (for markers mapped on crosses with few markers in common with example, Hlcs and Hgd). The major change from previous years is other crosses. Markers with a confidence of 3 are assigned a spe- that 56 of the new markers, more than half, have been localized on cific position due to constraints in presentation, but should be physical maps. This reflects efforts to physically map two regions assumed only to fall in the general region where they are posi- of the chromosome, the region with shared linkage on human Chr tioned. 22 (the D16Ais markers) and the distal part of the chromosome One hundred eleven of the markers on mouse Chr16 now have with shared linkage on human Chr 21 (the D16Jhu markers). These known human homologues. The regions of conserved synteny with projects have also mapped a number of existing markers to higher human chromosomes have been extended slightly in two cases. precision on physical maps, for example Comt and Erg. STCH, the proximal-most known gene on human Chr 21, extends The positions assigned here to the physically mapped markers the shared homology with mouse Chr 16 proximally, and show order and approximate physical distances, but are not to D16Xrf306, though not well-localized, is now the proximal-most precise scale. The regions being physically mapped reflect interest gene in the human Chr 16 region. Physical mapping demonstrates in using the mouse for models of human disease; in these cases, the that D16Mit28 and 29, distal to Dv13 by recombinational mapping, diseases of interest being the DiGeorge and Down syndromes. lie proximal to Thpo, making Dvl3 the proximal-most gene in the Fifteen new genes have been mapped on Chr 16 in the past human Chr 3 region. Eif4b at position 14.2 has a supposed human year as well as 31 new expressed sequence tags, most from the homologue on 18p11.2, but until the human mapping is confirmed ERATO Doi effort in Japan (the D16Ertd markers). The availabil- and other genes from human Chr 18 mapped on mouse Chr 16, this ity of radiation hybrid mapping panels for mouse should allow cannot be considered a new region of shared linkage with Chr 16. more rapid mapping of molecular markers in the future. New genes The regions of shared homologies are: a) human Chr 16p13: from found in regions with physical coverage can also be very easily D16Xrf306 at 1.7 to Ntan at 8.7; b) human Chr 8q11: Cebpd at 9 and accurately mapped. to Slugh at 9.4; c) human Chr 22q11.2: Vpreb1 at 9.8 to Igl-C1 at The rationale for marker localization and confidence assign- 13; d) human Chr 3q28q-13.3: Dvl3 at 13.1 to Pit1 at 43.5; and e) ments on the Committee Report map has been explained at length human Chr 21q11.2-q22.3: Stch at 47.2 to Mx2 at 69.7. In addi- in previous reports. Briefly, it is challenging to integrate markers tion, the first marker from rat, D16R11Mgh5, appears on the Chr from different crosses that have few markers in common. Markers 16 map at position 43. Six new traits appear on the map for a total that are placed with reasonable certainty in relation to flanking of 21: Tlsr5, Epd1, wsnp, nct, Cplaq2, and lpd1 and lpd2, which markers, or are non-recombinant with such markers, are indicated are counted as a single trait as they define the ends of a rearrange- by a confidence rating of 1. Markers mapped on well-characterized ment producing the lipid defect. This number will likely increase physical maps are precisely ordered and received a confidence in the future as loci identified in large-scale ENU mutagenesis rating of 1. Markers mapped on crosses with fewer commonly used projects are mapped. markers lie in the general region around the given position, but The Chr 16 map has been expanded this year from 70 to 72 order with respect to all flanking markers cannot be determined. map units. This is to better resolve orders of markers mapped on high density physical maps. * Committee Chair ** Co-Chair Acknowledgments. This work was supported in part by PHS awards Correspondence to: R.H. Reeves HD24605 and HG00405.
Mammalian Genome – Springer Journals
Published: Oct 1, 1999
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