A 7-bp deletion in the Cd4 gene, present in the strain MOLF/Ei of Mus musculus molossinus and absent in laboratory mouse strains (Mus musculus musculus), provided the means to distinguish the parental origin of the Cd4 alleles expressed in single cells of F1 (AKR × MOLF/Ei) and F1 (Balb/C × MOLF/Ei) hybrids. Single-cell RT-PCR showed that the individual CD4+ lymphocyte expresses either the maternal or the paternal Cd4 allele, never both. In situ hybridization proved that Cd4 alleles replicate asynchronously, as expected in the case of genes expressed monoallelically.
Mammalian Genome – Springer Journals
Published: Jan 1, 2004
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