Molecularly imprinted polymers for selective solid-phase extraction of phospholipids from human milk samples

Molecularly imprinted polymers for selective solid-phase extraction of phospholipids from human... The authors describe a method for the extraction and determination of phospholipids (PLs) from human milk fat by using a molecularly imprinted polymer (MIP) as the sorbent material and liquid chromatography for detection. The MIP was synthesized by thermal polymerization using L-α-phosphatidylcholine (PC) as the template, methacrylic acid as the functional monomer, ethylene glycol dimethacrylate as the cross-linker, and acetonitrile as the porogenic solvent. The resulting MIP was employed as a sorbent for solid-phase extraction of PLs from human milk fat samples. The extracted PLs were then determined by means of hydrophilic interaction LC in combination with evaporative light scattering detection. Variables affect the extraction efficiency (such as the porogenic solvent, template/functional monomer/cross-linker ratio and extraction conditions) were optimized. The selectivity of the MIP over PLs different from PC was investigated, and the adsorption capacity, breakthrough volume and reusability of the MIP was determined. The MIP displays a satisfactory loading capacity (ca. 41 μg PC per g of sorbent) and a high affinity and selectivity for PC and structurally related PLs, such as sphingomyelin and L-α-phosphatidylethanolamine. The MIP can be reused at least 20 times in case of PL standards, and at least 6 times in case of human milk fat samples. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Microchimica Acta Springer Journals

Molecularly imprinted polymers for selective solid-phase extraction of phospholipids from human milk samples

Loading next page...
 
/lp/springer_journal/molecularly-imprinted-polymers-for-selective-solid-phase-extraction-of-hU8YFRI0nh
Publisher
Springer Vienna
Copyright
Copyright © 2017 by Springer-Verlag Wien
Subject
Chemistry; Nanochemistry; Nanotechnology; Characterization and Evaluation of Materials; Analytical Chemistry; Microengineering
ISSN
0026-3672
eISSN
1436-5073
D.O.I.
10.1007/s00604-017-2345-6
Publisher site
See Article on Publisher Site

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches

$49/month

Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.

$588

$360/year

billed annually
Start Free Trial

14-day Free Trial