Molecular sex identification of sable and stone marten

Molecular sex identification of sable and stone marten Sable (Martes zibellina) and stone marten (Martes foina) populations are declining sharply and both species are threatened in China. Knowing the sex ratio of a population is important for long-term conservation and development of management plans for endangered marten species. We here describe the application of a novel PCR-RFLP method for sex determination in these two marten species. We developed a new designed primer pair (ZFLB1 and ZFLB2) that yields a smaller ZFX/ZFY PCR product (246 bp), which in combination with RFLP analysis, provides a simple and reliable method for gender determination in Martes zibellina and Martes foina. The method can be used on tissue and non-invasively collected hair samples for sex identification in wild marten populations, and is powerful for monitoring population status and trend of endangered martens. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Ecology Springer Journals

Molecular sex identification of sable and stone marten

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Publisher
SP MAIK Nauka/Interperiodica
Copyright
Copyright © 2013 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Ecology; Environment, general
ISSN
1067-4136
eISSN
1608-3334
D.O.I.
10.1134/S1067413613020148
Publisher site
See Article on Publisher Site

Abstract

Sable (Martes zibellina) and stone marten (Martes foina) populations are declining sharply and both species are threatened in China. Knowing the sex ratio of a population is important for long-term conservation and development of management plans for endangered marten species. We here describe the application of a novel PCR-RFLP method for sex determination in these two marten species. We developed a new designed primer pair (ZFLB1 and ZFLB2) that yields a smaller ZFX/ZFY PCR product (246 bp), which in combination with RFLP analysis, provides a simple and reliable method for gender determination in Martes zibellina and Martes foina. The method can be used on tissue and non-invasively collected hair samples for sex identification in wild marten populations, and is powerful for monitoring population status and trend of endangered martens.

Journal

Russian Journal of EcologySpringer Journals

Published: Mar 22, 2013

References

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