Molecular regulation of somatic embryogenesis in potato: an auxin led perspective

Molecular regulation of somatic embryogenesis in potato: an auxin led perspective Potato internodal segments (INS) treated with the auxin 2,4-dichlorophenoxyacetic acid can be induced to develop somatic embryos upon their transfer to an auxin-free medium, while the continuous presence of auxin in the medium suppresses the progression of embryogenically-induced somatic cells to embryos. We have employed these contrasting pathways, in combination with potato microarrays representing circa 10,000 genes, to profile global gene expression patterns during the progression of somatic embryogenesis in potato. The induction phase, characterised by the presence of auxin, was analysed by the direct comparison of RNA isolated from freshly excised (0 days) and embryogenically induced (14 days) INS explants. RNAs from embryo-forming (withdrawal of auxin after 14 days) and embryo-inhibitory (continuous presence of auxin) conditions, isolated over a range of time-points until the emergence of somatic embryos, were compared in a loop design to identify auxin responsive genes putatively involved in the process of somatic embryogenesis. A total of 402 transcripts were found to be showing significant differential expression patterns during somatic embryogenesis ‘induction’ phase, 524 during ‘embryo-transition’ phase, while 44 transcripts were common to both phases. Functional classification of these transcripts, using Gene Ontology vocabularies (molecular and biological), revealed that a significant proportion of transcripts were involved in processes which are more relevant to somatic embryogenesis such as apoptosis, development, reproduction, stress and signal transduction. This is the first study profiling global gene expression patterns during true somatic embryogenesis initiated from mature and completely differentiated explants and has enabled the description of stage-specific expression patterns of a large number of genes during potato somatic embryogenesis (PSE). The significance of the key identified genes during critical stages of somatic embryogenesis is discussed. Plant Molecular Biology Springer Journals

Molecular regulation of somatic embryogenesis in potato: an auxin led perspective

Loading next page...
Springer Netherlands
Copyright © 2008 by Springer Science+Business Media B.V.
Life Sciences; Plant Pathology; Biochemistry, general; Plant Sciences
Publisher site
See Article on Publisher Site


You’re reading a free preview. Subscribe to read the entire article.

DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches


Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.



billed annually
Start Free Trial

14-day Free Trial