Molecular regulation of somatic embryogenesis in potato: an auxin led perspective

Molecular regulation of somatic embryogenesis in potato: an auxin led perspective Potato internodal segments (INS) treated with the auxin 2,4-dichlorophenoxyacetic acid can be induced to develop somatic embryos upon their transfer to an auxin-free medium, while the continuous presence of auxin in the medium suppresses the progression of embryogenically-induced somatic cells to embryos. We have employed these contrasting pathways, in combination with potato microarrays representing circa 10,000 genes, to profile global gene expression patterns during the progression of somatic embryogenesis in potato. The induction phase, characterised by the presence of auxin, was analysed by the direct comparison of RNA isolated from freshly excised (0 days) and embryogenically induced (14 days) INS explants. RNAs from embryo-forming (withdrawal of auxin after 14 days) and embryo-inhibitory (continuous presence of auxin) conditions, isolated over a range of time-points until the emergence of somatic embryos, were compared in a loop design to identify auxin responsive genes putatively involved in the process of somatic embryogenesis. A total of 402 transcripts were found to be showing significant differential expression patterns during somatic embryogenesis ‘induction’ phase, 524 during ‘embryo-transition’ phase, while 44 transcripts were common to both phases. Functional classification of these transcripts, using Gene Ontology vocabularies (molecular and biological), revealed that a significant proportion of transcripts were involved in processes which are more relevant to somatic embryogenesis such as apoptosis, development, reproduction, stress and signal transduction. This is the first study profiling global gene expression patterns during true somatic embryogenesis initiated from mature and completely differentiated explants and has enabled the description of stage-specific expression patterns of a large number of genes during potato somatic embryogenesis (PSE). The significance of the key identified genes during critical stages of somatic embryogenesis is discussed. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

Molecular regulation of somatic embryogenesis in potato: an auxin led perspective

Loading next page...
 
/lp/springer_journal/molecular-regulation-of-somatic-embryogenesis-in-potato-an-auxin-led-E1Gtnh10Op
Publisher
Springer Journals
Copyright
Copyright © 2008 by Springer Science+Business Media B.V.
Subject
Life Sciences; Plant Pathology; Biochemistry, general; Plant Sciences
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1007/s11103-008-9360-2
Publisher site
See Article on Publisher Site

Abstract

Potato internodal segments (INS) treated with the auxin 2,4-dichlorophenoxyacetic acid can be induced to develop somatic embryos upon their transfer to an auxin-free medium, while the continuous presence of auxin in the medium suppresses the progression of embryogenically-induced somatic cells to embryos. We have employed these contrasting pathways, in combination with potato microarrays representing circa 10,000 genes, to profile global gene expression patterns during the progression of somatic embryogenesis in potato. The induction phase, characterised by the presence of auxin, was analysed by the direct comparison of RNA isolated from freshly excised (0 days) and embryogenically induced (14 days) INS explants. RNAs from embryo-forming (withdrawal of auxin after 14 days) and embryo-inhibitory (continuous presence of auxin) conditions, isolated over a range of time-points until the emergence of somatic embryos, were compared in a loop design to identify auxin responsive genes putatively involved in the process of somatic embryogenesis. A total of 402 transcripts were found to be showing significant differential expression patterns during somatic embryogenesis ‘induction’ phase, 524 during ‘embryo-transition’ phase, while 44 transcripts were common to both phases. Functional classification of these transcripts, using Gene Ontology vocabularies (molecular and biological), revealed that a significant proportion of transcripts were involved in processes which are more relevant to somatic embryogenesis such as apoptosis, development, reproduction, stress and signal transduction. This is the first study profiling global gene expression patterns during true somatic embryogenesis initiated from mature and completely differentiated explants and has enabled the description of stage-specific expression patterns of a large number of genes during potato somatic embryogenesis (PSE). The significance of the key identified genes during critical stages of somatic embryogenesis is discussed.

Journal

Plant Molecular BiologySpringer Journals

Published: Jun 16, 2008

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off