The Gy2 and Gy4 genes encoding glycinin subunits were molecularly manipulated and modified to test the possibility of increasing the nutritional value of soybean seed proteins. With the respective recombinant DNAs (pSP65/G2HG4, pSP65/G4HG2, pSP65/248Metl, pSP65/248Met2,3, and pSP65/248Metl,2,3), the in vitro translation was used to produce (i) chimeric proteins consisting of reciprocally exchanged acidic and basic domains of the G2 and G4 subunits, and (ii) G4 point mutants with increased number of methionine residues. The ability of the recombinant proteins to assemble into proper quaternary structures was inspected by sucrose gradient fractionation. The produced data could provide valuable clues for the potential improvement of the genetically modified crops.
Russian Journal of Plant Physiology – Springer Journals
Published: May 19, 2005
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
All the latest content is available, no embargo periods.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud