Molecular identification of Mecistocirrus digitatus and Toxocara vitulorum in the mithun (Bos frontalis) from north-east India

Molecular identification of Mecistocirrus digitatus and Toxocara vitulorum in the mithun (Bos... Mecistocirrus digitatus and Toxocara vitulorum are common pathogenic nematode parasites of mithun (Bos frontalis). Species identification by morphological features was confirmed by molecular identification of M. digitatus and T. vitulorum. The internal transcribed spacer-2 (ITS-2) region and beta tubulin gene of M. digitatus were polymerase chain reaction (PCR) amplified and sequenced. ITS-2 sequence analysis showed 100% homology with other isolates of M. digitatus and 83% identity with Haemonchus contortus and H. placei, respectively. Likewise, ITS-1 and ITS-2 sequences of T. vitulorum were PCR amplified and sequenced. Sequence analysis of these internal transcribed spacers from five worms of the parasite from mithun showed no intraspecific variations with T. vitulorum isolates from domestic ruminants. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Parasitic Diseases Springer Journals

Molecular identification of Mecistocirrus digitatus and Toxocara vitulorum in the mithun (Bos frontalis) from north-east India

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Publisher
Springer India
Copyright
Copyright © 2017 by Indian Society for Parasitology
Subject
Medicine & Public Health; Infectious Diseases; Health Promotion and Disease Prevention; Medicine/Public Health, general
ISSN
0971-7196
eISSN
0975-0703
D.O.I.
10.1007/s12639-017-0879-5
Publisher site
See Article on Publisher Site

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