Molecular identification of GAPDHs in cassava highlights the antagonism of MeGAPCs and MeATG8s in plant disease resistance against cassava bacterial blight

Molecular identification of GAPDHs in cassava highlights the antagonism of MeGAPCs and MeATG8s in... Key message MeGAPCs were identified as negative regulators of plant disease resistance, and theinteraction of MeGAPCs and MeATG8s was highlighted in plant defense response. Abstract As an important enzyme of glycolysis metabolic pathway, glyceraldehyde-3-P dehydrogenase (GAPDH) plays important roles in plant development, abiotic stress and immune responses. Cassava (Manihot esculenta) is most important tropical crop and one of the major food crops, however, no information is available about GAPDH gene family in cassava. In this study, 14 MeGAPDHs including 6 cytosol GAPDHs (MeGAPCs) were identified from cassava, and the transcripts of 14 MeGAPDHs in response to Xanthomonas axonopodis pv manihotis (Xam) indicated their possible involvement in immune responses. Further investigation showed that MeGAPCs are negative regulators of disease resistance against Xam. Through transient expression in Nicotiana benthamiana, we found that overexpression of MeGAPCs led to decreased disease resistance against Xam. On the contrary, MeGAPCs-silenced cassava plants through virus-induced gene silencing (VIGS) conferred improved disease resistance. Notably, MeGAPCs physically interacted with autophagy-related protein 8b (MeATG8b) and MeATG8e and inhibited autophagic activity. Moreover, MeATG8b and MeATG8e negatively regulated the activities of NAD- dependent MeGAPDHs, and are involved in MeGAPCs-mediated disease resistance. Taken together, this study highlights the involvement of MeGAPCs in http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

Molecular identification of GAPDHs in cassava highlights the antagonism of MeGAPCs and MeATG8s in plant disease resistance against cassava bacterial blight

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Publisher
Springer Journals
Copyright
Copyright © 2018 by Springer Science+Business Media B.V., part of Springer Nature
Subject
Life Sciences; Plant Sciences; Biochemistry, general; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1007/s11103-018-0733-x
Publisher site
See Article on Publisher Site

Abstract

Key message MeGAPCs were identified as negative regulators of plant disease resistance, and theinteraction of MeGAPCs and MeATG8s was highlighted in plant defense response. Abstract As an important enzyme of glycolysis metabolic pathway, glyceraldehyde-3-P dehydrogenase (GAPDH) plays important roles in plant development, abiotic stress and immune responses. Cassava (Manihot esculenta) is most important tropical crop and one of the major food crops, however, no information is available about GAPDH gene family in cassava. In this study, 14 MeGAPDHs including 6 cytosol GAPDHs (MeGAPCs) were identified from cassava, and the transcripts of 14 MeGAPDHs in response to Xanthomonas axonopodis pv manihotis (Xam) indicated their possible involvement in immune responses. Further investigation showed that MeGAPCs are negative regulators of disease resistance against Xam. Through transient expression in Nicotiana benthamiana, we found that overexpression of MeGAPCs led to decreased disease resistance against Xam. On the contrary, MeGAPCs-silenced cassava plants through virus-induced gene silencing (VIGS) conferred improved disease resistance. Notably, MeGAPCs physically interacted with autophagy-related protein 8b (MeATG8b) and MeATG8e and inhibited autophagic activity. Moreover, MeATG8b and MeATG8e negatively regulated the activities of NAD- dependent MeGAPDHs, and are involved in MeGAPCs-mediated disease resistance. Taken together, this study highlights the involvement of MeGAPCs in

Journal

Plant Molecular Biology – Springer Journals

Published: Apr 20, 2018

References

  • Plastidial glycolytic glyceraldehyde-3-phosphate dehydrogenase is an important determinant in the carbon and nitrogen metabolism of heterotrophic cells in Arabidopsis
    Anoman, AD; Muñoz-Bertomeu, J; Rosa-Téllez, S; Flores-Tornero, M; Serrano, R; Bueso, E; Fernie, AR; Segura, J; Ros, R
  • Reactive oxygen species: metabolism, oxidative stress, and signal transduction
    Apel, K; Hirt, H
  • Gene expression profile in response to Xanthomonas axonopodis pv manihotis infection in cassava using a cDNA microarray
    Camilo, L; Soto, M; Restrepo, S
  • Over-expression of PsGPD, a mushroom glyceraldehyde-3-phosphatedehydrogenase gene, enhances salt tolerance in rice plants
    Cho, JI; Lim, HM; Siddiqui, ZS; Park, SH; Kim, AR; Kwon, TR; Lee, SK; Park, SC; Jeong, MJ; Lee, GS
  • GAPDH and autophagy preserve survival after apoptotic cytochrome c release in the absence of caspase activation
    Colell, A; Ricci, JE; Tait, S; Milasta, S; Maurer, U; Bouchier-Hayes, L; Fitzgerald, P; Guio-Carrion, A; Waterhouse, NJ; Li, CW; Mari, B; Barbry, P; Newmeyer, DD; Beere, HM; Green, DR
  • Random GFP::cDNA fusions enable visualization of subcellular structures in cells of Arabidopsis at a high frequency
    Cutler, SR; Ehrhardt, DW; Griffitts, JS; Somerville, CR
  • An effector of the Irish potato famine pathogen antagonizes a host autophagy cargo receptor
    Dagdas, YF; Belhaj, K; Maqbool, A; Chaparro-Garcia, A; Pandey, P; Petre, B; Tabassum, N; Cruz-Mireles, N; Hughes, RK; Sklenar, J; Win, J; Menke, F; Findlay, K; Banfield, MJ; Kamoun, S; Bozkurt, TO
  • The Pfam protein families database: towards a more sustainable future
    Finn, RD; Coggill, P; Eberhardt, RY; Eddy, SR; Mistry, J; Mitchell, AL; Potter, SC; Punta, M; Qureshi, M; Sangrador-Vegas, A; Salazar, GA; Tate, J; Bateman, A
  • Cytosolic glyceraldehyde-3-phosphate dehydrogenases interact with phospholipase Dδ to transduce hydrogen peroxide signals in the Arabidopsis response to stress
    Guo, L; Devaiah, SP; Narasimhan, R; Pan, X; Zhang, Y; Zhang, W; Wang, X
  • Cytosolic phosphorylating glyceraldehyde-3-phosphate dehydrogenases affect Arabidopsis cellular metabolism and promote seed oil accumulation
    Guo, L; Ma, F; Wei, F; Fanella, B; Allen, DK; Wang, X
  • Selective autophagy limits cauliflower mosaic virus infection by NBR1-mediated targeting of viral capsid protein and particles
    Hafrén, A; Macia, JL; Love, AJ; Milner, JJ; Drucker, M; Hofius, D
  • Cytoplastic glyceraldehyde-3-phosphate dehydrogenases interact with ATG3 to negatively regulate autophagy and immunity in Nicotiana benthamiana
    Han, S; Wang, Y; Zheng, X; Jia, Q; Zhao, J; Bai, F; Hong, Y; Liu, Y
  • S-nitrosylated GAPDH initiates apoptotic cell death by nuclear translocation following Siah1 binding
    Hara, MR; Agrawal, N; Kim, SF; Cascio, MB; Fujimuro, M; Ozeki, Y; Takahashi, M; Cheah, JH; Tankou, SK; Hester, LD; Ferris, CD; Hayward, SD; Snyder, SH; Sawa, A
  • A Pseudomonas syringae type III effector suppresses cell wall-based extracellular defense in susceptible Arabidopsis plants
    Hauck, P; Thilmony, R; He, SY
  • Autophagy functions as an antiviral mechanism against geminiviruses in plants
    Haxim, Y; Ismayil, A; Jia, Q; Wang, Y; Zheng, X; Chen, T; Qian, L; Liu, N; Wang, Y; Han, S; Cheng, J; Qi, Y; Hong, Y; Liu, Y
  • Beyond glycolysis: GAPDHs are multi-functional enzymes involved in regulation of ROS, autophagy, and plant immune responses
    Henry, E; Fung, N; Liu, J; Drakakaki, G; Coaker, G
  • Jasmonate regulates the inducer of cbf expression-C-repeat binding factor/DRE binding factor1 cascade and freezing tolerance in Arabidopsis
    Hu, Y; Jiang, L; Wang, F; Yu, D
  • GSDS 2.0: an upgraded gene feature visualization server
    Hu, B; Jin, J; Guo, AY; Zhang, H; Luo, J; Gao, G
  • A ubiquitin-like system mediates protein lipidation
    Ichimura, Y; Kirisako, T; Takao, T
  • Resistance of Nicotiana benthamiana to Phytophthora infestans is mediated by the recognition of the elicitor protein INF1
    Kamoun, S; West, P; Vleeshouwers, VG; Groot, KE; Govers, F
  • Arabidopsis WRKY38 and WRKY62 transcription factors interact with histone deacetylase 19 in basal defense
    Kim, KC; Lai, Z; Fan, B; Chen, Z
  • Phosphatidic acid binds to cytosolic glyceraldehyde-3-phosphatedehydrogenase and promotes its cleavage in Arabidopsis
    Kim, SC; Guo, L; Wang, X
  • The reversible modification regulates the membrane-binding state of Apg8/Aut7 essential for autophagy and the cytoplasm to vacuole targeting pathway
    Kirisako, T; Ichimura, Y; Okada, H
  • The Rab GTPase RabG3b positively regulates autophagy and immunity-associated hypersensitive cell death in Arabidopsis
    Kwon, SI; Cho, HJ; Kim, SR; Park, OK
  • Proteomic identification of S-nitrosylated proteins in Arabidopsis
    Lindermayr, C; Saalbach, G; Durner, J
  • Virus-induced gene silencing in tomato
    Liu, Y; Schiff, M; Dinesh-Kumar, SP
  • CDD: NCBI’s conserved domain database
    Marchler-Bauer, A; Derbyshire, MK; Gonzales, NR; Lu, S; Chitsaz, F; Geer, LY; Geer, RC; He, J; Gwadz, M; Hurwitz, DI; Lanczycki, CJ; Lu, F; Marchler, GH; Song, JS; Thanki, N; Wang, Z; Yamashita, RA; Zhang, D; Zheng, C; Bryant, SH
  • Posttranslational modification of glyceraldehyde-3-phosphate dehydrogenase by S-nitrosylation and subsequent NADH attachment
    Mohr, S; Stamler, JS; Brüne, B
  • Plastidial glyceraldehyde-3-phosphate dehydrogenase deficiency leads to altered root development and affects the sugar and amino acid balance in Arabidopsis
    Muñoz-Bertomeu, J; Cascales-Miñana, B; Mulet, JM; Baroja-Fernández, E; Pozueta-Romero, J; Kuhn, JM; Segura, J; Ros, R
  • The plastidial glyceraldehyde-3-phosphate dehydrogenase is critical for viable pollen development in Arabidopsis
    Muñoz-Bertomeu, J; Cascales-Miñana, B; Irles-Segura, A; Mateu, I; Nunes-Nesi, A; Fernie, AR; Segura, J; Ros, R
  • Arabidopsis plants deficient in plastidial glyceraldehyde-3-phosphatedehydrogenase show alterations in abscisic acid (ABA) signal transduction: interaction between ABA and primary metabolism
    Muñoz-Bertomeu, J; Bermúdez, MA; Segura, J; Ros, R
  • RNAseq analysis of cassava reveals similar plant responses upon infection with pathogenic and non-pathogenic strains of Xanthomonas axonopodis pv. manihotis
    Muñoz-Bodnar, A; Perez-Quintero, AL; Gomez-Cano, F; Gil, J; Michelmore, R; Bernal, A; Szurek, B; Lopez, C
  • Cloning of a peroxidase Gene from cassava with potential as a molecular marker for resistance to bacterial blight
    Pereiral, LP; Goodwin, PH; Erickson, L
  • Origin, evolution, and metabolic role of a novel glycolytic GAPDH enzyme recruited by land plant plastids
    Petersen, J; Brinkmann, H; Cerff, R
  • The organization and regulation of plant glycolysis
    Plaxton, WC
  • Identification of ta-siRNAs and cis-nat-siRNAs in cassava and their roles in response tocassava bacterial blight
    Quintero, A; Pérez-Quintero, AL; López, C
  • Characterization of an Arabidopsis thaliana mutant lacking a cytosolic non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase
    Rius, SP; Casati, P; Iglesias, AA; Gomez-Casati, DF
  • Characterization of Arabidopsis lines deficient in GAPC-1, a cytosolic NAD-dependent glyceraldehyde-3-phosphate dehydrogenase
    Rius, SP; Casati, P; Iglesias, AA; Gomez-Casati, DF
  • GOSPEL: a neuroprotective protein that binds to GAPDH upon S-Nitrosylation
    Sen, N; Hara, MR; Ahmad, AS; Cascio, MB; Kamiya, A; Ehmsen, JT; Agrawal, N; Hester, L; Doré, S; Snyder, SH; Sawa, A
  • Exogenous application of hydrogen sulfide donor sodium hydrosulfide enhanced multiple abiotic stress tolerance in bermudagrass (Cynodon dactylon (L). Pers.)
    Shi, H; Ye, T; Chan, Z
  • New nuclear functions of the glycolytic protein, glyceraldehyde-3-phosphate dehydrogenase, in mammalian cells
    Sirover, MA
  • Rapid, transient expression of fluorescent fusion proteins in tobacco plants and generation of stably transformed plants
    Sparkes, IA; Runions, J; Kearns, A; Hawes, C
  • The impact of oxidative stress on Arabidopsis mitochondria
    Sweetlove, LJ; Heazlewood, JL; Herald, V; Holtzapffel, R; Day, DA; Leaver, CJ; Millar, AH
  • MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods
    Tamura, K; Peterson, D; Peterson, N; Stecher, G; Nei, M; Kumar, S
  • Nuclear accumulation of cytosolic glyceraldehyde-3-phosphatedehydrogenase in cadmium-stressed Arabidopsis roots
    Vescovi, M; Zaffagnini, M; Festa, M; Trost, P; Lo Schiavo, F; Costa, A
  • Tomato HsfA1a plays a critical role in plant drought tolerance by activating ATG genes and inducing autophagy
    Wang, Y; Cai, S; Yin, L; Shi, K; Xia, X; Zhou, Y; Yu, J; Zhou, J
  • Two transcriptional activators of N-acetylserotonin O-methyltransferase 2 and melatonin biosynthesis in cassava
    Wei, Y; Liu, G; Bai, Y; Xia, F; He, C; Shi, H
  • MeWRKY20 and its interacting and activating autophagy-related protein 8 (MeATG8) regulate plant disease resistance in cassava
    Yan, Y; Wang, P; He, C; Shi, H
  • Stress responses and metabolic regulation of glyceraldehyde-3-phosphate dehydrogenase genes in Arabidopsis
    Yang, Y; Kwon, HB; Peng, HP; Shih, MC
  • Mechanisms of nitrosylation and denitrosylation of cytoplasmic glyceraldehyde-3-phosphate dehydrogenase from Arabidopsis thaliana
    Zaffagnini, M; Morisse, S; Bedhomme, M; Marchand, CH; Festa, M; Rouhier, N; Lemaire, SD; Trost, P
  • Genome-wide identification and characterization of glyceraldehyde-3-phosphate dehydrogenase genes family in wheat (Triticum aestivum)
    Zeng, L; Deng, R; Guo, Z; Yang, S; Deng, X
  • Overexpression of a cytosolic glyceraldehyde-3-phosphate dehydrogenase gene OsGAPC3 confers salt tolerance in rice
    Zhang, XH; Rao, XL; Shi, HT; Li, RJ; Lu, YT
  • NBR1-mediated selective autophagy targets insoluble ubiquitinated protein aggregates in plant stress responses
    Zhou, J; Wang, J; Cheng, Y; Chi, YJ; Fan, B; Yu, JQ; Chen, Z

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