ISSN 1022-7954, Russian Journal of Genetics, 2008, Vol. 44, No. 8, pp. 944–947. © Pleiades Publishing, Inc., 2008.
Original Russian Text © A.V. Nedoluzhko, A.V. Tikhonov, D.B. Dorokhov, 2008, published in Genetika, 2008, Vol. 44, No. 8, pp. 1084–1088.
Due to increasing anthropogenic impact on natural
environment, the biodiversity conservation of wild spe-
cies, closely related to cultivated plants, becomes an
urgent problem. Conservation of the gene pools of the
species related to cultivated plants reserves the possi-
bility of the involvement of their genes into breeding
programs, realized with the help of traditional
approaches, as well as with the modern methods of bio-
Anthropogenic inﬂuence can destroy the habitats of
many species, change natural ecosystems and genetic
diversity of wild plants. Moreover, in some cases it can
cause extinction of the plant species. At the same time,
for some species, anthropogenic landscapes have
become normal habitats.
It is considered that one of most important conse-
quences of anthropogenic inﬂuence on the species
range structure is its fragmentation into more or less
isolated fragments . In case of wild soybean, the
nearest relative of cultivated soybean
Merr., the situation is somewhat different. Wild soy-
bean is a ruderal weed, which prefers disturbed phyto-
cenoses. In natural landscapes (river valleys, banks of
the river islands, canals, former river-beds, regenerat-
ing meadows, hill slopes, and scree) 
number of seed dispersal mechanisms, including auto-
chory, zoochory, and hydrochory. The latter mechanism
becomes important for the range extension in river val-
leys, since in case of autochory the seeds are dispersed
around the mother plant at a distance from 1 to 3 m.
Wild soybean uses human-disturbed phytocenoses.
These are mostly roadsides, which are used as artiﬁcial
corridors. Economical activity does not shrink the
range; on the contrary, it leads to the range
Apparently, the advantages of roads as factors pro-
viding the range extension should lead to the change of
the wild soybean population genetic structure due to
possible admixture of different gene pools.
We examined biodiversity of wild soybean during
numerous expeditions to the
center of origin in
the Far East of Russia. The sites of contacts of natural
and anthropogenic cenoses with
edly described. In most of the cases, these were the
intersections of roads with riverbeds. In this respect,
detailed molecular genetic investigations of the wild
soybean populations at the sites of contacts and possi-
ble interaction between the
natural and anthropogenic cenoses deserve special
interest [3, 4].
Comparative analysis of the population genetic
structures of natural and anthropogenic landscapes can
provide important information on the changes taking
place in natural populations of
and to elaborate
the measures for conservation of the unique natural
gene bank. The use of wild soybean seeds in breeding
programs of cultivated soybean will provide speedy
generation of highly productive cultivars .
Wild soybean has been studied for a long period of
time with the help of different methods, including
molecular markers. The latter approach is used for the
analysis of wild soybean origin [6, 7], phylogeny
10], and population genetic structure [11–18]. At
the same time, the studies devoted to detailed analysis
of the genetic structure of individual wild soybean pop-
ulations growing in different ecological conditions, and
the relationships between these populations are
extremely rare and sporadic [14, 18].
Molecular Genetic Analysis of Wild Soybean (
Sieb. & Zucc.) Population Structure in Anthropogenic
and Natural Landscapes of Primorskii Krai
A. V. Nedoluzhko, A. V. Tikhonov, and D. B. Dorokhov
Bioengineering Center, Russian Academy of Sciences, Moscow, 117312 Russia
Received February 12, 2008
—The data are presented on genetic population structure of wild soybean growing in natural and
anthropogenically disturbed landscapes of Primorskii krai of the Russian Federation. Comparative analysis
showed that wild soybean populations exposed to anthropogenic inﬂuence exhibited lower genetic diversity
than natural populations. Recommendations on conservation of the wild plant gene pools using comparative
data on population genetic structures are made.