Molecular Genetic Analysis of the Drosophila melanogaster Trithorax-likeGene Coding for the GAGA Transcription Factor

Molecular Genetic Analysis of the Drosophila melanogaster Trithorax-likeGene Coding for the GAGA... The Drosophila melanogaster Trithorax-like(Trl) gene is classed with thetrx-Ggenes and codes for several isoforms of the GAGA transcription factor (GAF) which regulates expression of homeotic and numerous other genes. GAF acts as a transcriptional antirepressor, i.e., its interaction with nucleosomal DNA results in the open chromatin conformation in promoter gene regions. The regions thereby become accessible to other transcription factors. As mutations of theTrlgene enhance position effect variegation and disturb chromosome segregation in mitosis and meiosis, GAF is thought to play another, more significant role in determining the chromatin structure. To study the molecular basis of its pleiotropic effect, the Trlgene was subjected to a structural analysis. The genomic fragment encompassing Trlgene was sequenced, the sizes of its exons and introns was established, and a complex structure of the 5" and 3" gene regions was demonstrated. The Trl 13C, Trl 62, DfTrl R67, and DfTrl R85mutations were exactly mapped. In addition, four insertions of the Pelement were identified as Trlalleles (Trl l(3)s2325, Trl EP(3)3184, Trl EP(3)3191, and Trl EP(3)3609). The viability at various developmental stages was studied in homozygotes for the Trlmutations and in interallelic compounds. The following lethality stages were established: hatching, (Trl 13C, DfTrl R85, Trl EP(3)3609), larval molts (Trl l(3)s2325), pupation, metamorphosis (DfTrl R67, Trl 62), and eclosion (some compounds). Russian Journal of Genetics Springer Journals

Molecular Genetic Analysis of the Drosophila melanogaster Trithorax-likeGene Coding for the GAGA Transcription Factor

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Kluwer Academic Publishers-Plenum Publishers
Copyright © 2001 by MAIK “Nauka/Interperiodica”
Biomedicine; Human Genetics
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