Molecular dissection of novel anthocyanin transcripts HsCHS and HsF3H from the calyx tissue of Hibiscus sabdariffa L.

Molecular dissection of novel anthocyanin transcripts HsCHS and HsF3H from the calyx tissue of... Flavonoids are polyphenolic compounds commonly found in vegetables as well as fruits and contribute significantly in the human diet. The calyx of roselle (Hibiscus sabdariffa L.) is rich in polyphenolic compounds and anthocyanins. Chalcone synthase (CHS) and flavanone 3-hydroxylase (F3H) are two important genes involved in the biosynthesis of flavonoids including anthocyanins in plants. The two transcripts designated as HsCHS (KR709156) and HsF3H (KR709157) were isolated from the calyx tissue of roselle using the Rapid Amplification of cDNA Ends PCR and PCR walking approaches, which encoded the polypeptides of 389 and 368 amino acids, respectively. Several important domains were revealed in the HsCHS amino acid sequence, including CHS-like, fabH, BcsA, Chal-sti-synt-N and Chal-sti-synt-C, which indicates that the isolated gene is probably a CHS belonging to the polyketide synthase family. On the other hand, identification of 2OG-FeII_oxy, Isopenicillin N synthase-like, DIOXN and PLN02515 domains in HsF3H protein sequence supports the idea that the isolated gene is an F3H related to the large gene family of 2-oxoglutarate-dependent dioxygenases. This study predicted the putative functions of the two central genes governing the flavonoid pathway in H. sabdariffa, which leads to anthocyanin production. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png International Journal of Environmental Science and Technology Springer Journals

Molecular dissection of novel anthocyanin transcripts HsCHS and HsF3H from the calyx tissue of Hibiscus sabdariffa L.

Download PDF
12 pages
Read Article

Loading next page...
 
/lp/springer_journal/molecular-dissection-of-novel-anthocyanin-transcripts-hschs-and-hsf3h-G0ulP0HfJS
Publisher
Springer Journals
Copyright
Copyright © 2018 by Islamic Azad University (IAU)
Subject
Environment; Environment, general; Environmental Science and Engineering; Environmental Chemistry; Waste Water Technology / Water Pollution Control / Water Management / Aquatic Pollution; Soil Science & Conservation; Ecotoxicology
ISSN
1735-1472
eISSN
1735-2630
DOI
10.1007/s13762-018-1762-3
Publisher site
See Article on Publisher Site

Abstract

Flavonoids are polyphenolic compounds commonly found in vegetables as well as fruits and contribute significantly in the human diet. The calyx of roselle (Hibiscus sabdariffa L.) is rich in polyphenolic compounds and anthocyanins. Chalcone synthase (CHS) and flavanone 3-hydroxylase (F3H) are two important genes involved in the biosynthesis of flavonoids including anthocyanins in plants. The two transcripts designated as HsCHS (KR709156) and HsF3H (KR709157) were isolated from the calyx tissue of roselle using the Rapid Amplification of cDNA Ends PCR and PCR walking approaches, which encoded the polypeptides of 389 and 368 amino acids, respectively. Several important domains were revealed in the HsCHS amino acid sequence, including CHS-like, fabH, BcsA, Chal-sti-synt-N and Chal-sti-synt-C, which indicates that the isolated gene is probably a CHS belonging to the polyketide synthase family. On the other hand, identification of 2OG-FeII_oxy, Isopenicillin N synthase-like, DIOXN and PLN02515 domains in HsF3H protein sequence supports the idea that the isolated gene is an F3H related to the large gene family of 2-oxoglutarate-dependent dioxygenases. This study predicted the putative functions of the two central genes governing the flavonoid pathway in H. sabdariffa, which leads to anthocyanin production.

Journal

International Journal of Environmental Science and Technology – Springer Journals

Published: May 28, 2018

References

  • Insect pests and predators inhabiting roselle plants, Hibiscus sabdariffa L., a medicinal plant in Egypt
    Abdel-Moniem, ASH; Abd El-Wahab, TE
  • Structure and function of the chalcone synthase superfamily of plant type III polyketide synthases
    Abe, I; Morita, H
  • Electrochemical sensor based on graphene oxide/iron nanoparticles for the analysis of quercetin
    Akyıldırım, O; Medetalibeyoğlu, H; Manap, S; Beytur, M; Tokalı, FS; Yola, ML; Atar, N
  • The DNA-repair protein AlkB, EGL-9, and leprecan define new families of 2-oxoglutarate- and iron-dependent dioxygenases
    Aravind, L; Koonin, EV
  • The chalcone synthase superfamily of type III polyketide synthases
    Austin, MB; Noel, JP
  • Commercial practice of roselle (Hibiscus sabdariffa L.) beverage production: Optimization of hot water extraction and sweetness level
    Bolade, MK; Oluwalana, IB; Ojo, O
  • Molecular characterization of flavanone 3-hydroxylases consensus sequence, comparison with related enzymes and the role of conserved histidin residues
    Britschi, L; Dedio, J; Saedler, H; Forkmann, G
  • Synthesis, characterization and chemical studies of Hibiscus sabdariffa-g-copolymers
    Chauhan, A; Kaith, B
  • Thermal degradation kinetics of anthocyanins from blood orange, blackberry, and roselle using the arrhenius, eyring, and ball models
    Cisse, M; Vaillant, F; Acosta, O; Dhuique-Mayer, C; Dornier, M
  • Cloning and characterization of two members of the chalcone synthase gene family from walnut
    Claudot, AC; Ernst, D; Sandermann, H; Drouet, A
  • A new member of the chalcone synthase (CHS) family in sugarcane
    Contessotto, MGG; Monteiro-vitorello, CB; Mariani, PDSC; Coutinho, LL
  • Chalcone synthase and its functions in plant resistance
    Dao, TTH; Linthorst, HJM; Verpoorte, R
  • Allele-specific assay reveals functional variation in the chalcone synthase promoter of Arabidopsis thaliana that is compatible with neutral evolution
    Meaux, J; Goebel, U; Pop, A; Mitchell-Olds, T
  • Isolation of plant DNA from fresh tissue
    Doyle, JJ; Doyle, JL
  • Highly selective and sensitive voltammetric sensor based on ruthenium nanoparticle anchored calix[4]amidocrown-5 functionalized reduced graphene oxide: simultaneous determination of quercetin, morin and rutin in grape wine
    Elçin, S; Yola, ML; Eren, T; Girgin, B; Atar, N
  • Structure of chalcone synthase and the molecular basis of plant polyketide biosynthesis
    Ferrer, JL; Jez, JM; Bowman, ME; Dixon, RA; Noel, JP
  • A novel molecular imprinted nanosensor based quartz crystal microbalance for determination of kaempferol
    Gupta, VK; Yola, ML; Atar, N
  • Differential expression of three flavanone 3-hydroxylase genes in grains and coleoptiles of wheat
    Himi, E; Maekawa, M; Noda, K
  • Genetics and biochemistry of ant hocyanin biosynthesis
    Holton, TA; Cornish, EC
  • Divergent evolution of the thiolase superfamily and chalcone synthase family
    Jiang, C; Kim, SY; Suh, DY
  • Flavonoid biosynthesis. A colorful model for genetics
    Lepiniec, L; Debeaujon, I; Routaboul, J; Baudry, A; Pourcel, L; Nesi, NMC
  • Tailoring gut immune responses with lipoteichoic acid-deficient Lactobacillus acidophilus
    Lightfoot, YL; Mohamadzadeh, M
  • Plant physiology and biochemistry regulation of fl avanone 3-hydroxylase gene involved in the flavonoid biosynthesis pathway in response to UV-B radiation and drought stress in the desert plant, Reaumuria soongorica
    Liu, M; Li, X; Liu, Y; Cao, B
  • Gene identification in novel eukaryotic genomes by self-training algorithm
    Lomsadze, A
  • Multifunctional flavonoid dioxygenases: flavonol and anthocyanin biosynthesis in Arabidopsis thaliana L
    Martens, S; Preuß, A; Matern, U
  • Roselle (Hibiscus sabdariffa L.) oil as an alternative feedstock for biodiesel production in Thailand
    Nakpong, P; Wootthikanokkhan, S
  • Biochemical and genetic characterization of Arabidopsis flavanone 3 b-hydroxylase
    Owens, DK; Crosby, KC; Runac, J; Howard, BA; Winkel, BSJ
  • The neighbor-joining method: a new method for reconstructing phylogenetic trees
    Saitou, N; Nei, M
  • Studies on biological activities and phytochemicals composition of Hibiscus species—a review
    Salem, MZM; Olivares-Pérez, J; Salem, AZM
  • A review of phytochemistry and pharmacology of flavonoids
    Sandhar, H; Kumar, B; Prasher, S; Tiwari, P
  • Expression patterns of novel genes encoding homeodomain leucine-zipper proteins in Arabidopsis thaliana
    Sderman, E; Mattsson, J; Svenson, M; Borkird, C; Engstrm, P
  • Informatics and management science I
    She, H; He, S; Zhou, Z; Zhang, Q
  • Cloning and characterization of a Flavanone 3-hydroxylase gene from Ginkgo biloba
    Shen, G; Pang, AY; Wu, AW; Sun, X; Tang, AK
  • Functional characterization of key structural genes in rice flavonoid biosynthesis
    Shih, CH; Chu, H; Tang, LK; Sakamoto, W; Maekawa, M; Chu, IK; Lo, C
  • Identification of amino acid residues important in the cyclization reactions of chalcone and stilbene synthases
    Suh, DY; Fukuma, K; Kagami, J; Yamazaki, Y; Shibuya, M; Ebizuka, Y; Sankawa, U
  • MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods
    Tamura, K; Peterson, D; Peterson, N; Stecher, G; Nei, M; Kumar, S
  • Intra-specific hybridization: Generator of genetic diversification and heterosis in Andrographis paniculata Nees. A bridge from extinction to survival
    Valdiani, A; Kadir, MA; Saad, MS; Talei, D; Tan, SG
  • Microsatellite-based evidences of genetic bottlenecks in the cryptic species “Andrographis paniculata Nees”: a potential anticancer agent
    Valdiani, A; Javanmard, A; Talei, D; Tan, SG; Nikzad, S; Kadir, MA; Abdullah, SNA
  • Genoproteomics-assisted improvement of Andrographis paniculata: toward a promising molecular and conventional breeding platform for autogamous plants affecting pharmaceutical industry
    Valdiani, A; Talei, D; Lattoo, SK; Ortiz, R; Rasmussen, SK; Batley, J; Rafii, MY; Maziah, M; Sabu, KK; Abiri, R; Sakuanrungsirikul, S; Tan, SG
  • Cloning and characterization of chalcone synthase gene from Pueraria candollei var. mirifica
    Wiriyaampaiwong, P; Thanonkeo, S; Thanonkeo, P
  • A novel voltammetric sensor based on gold nanoparticles involved in p-aminothiophenol functionalized multi-walled carbon nanotubes: application to the simultaneous determination of quercetin and rutin
    Yola, ML; Atar, N
  • A novel voltammetric sensor based on p-aminothiophenol functionalized graphene oxide/gold nanoparticles for determining quercetin in the presence of ascorbic acid
    Yola, ML; Atar, N; Üstündağ, Z; Solak, AO
  • A novel electro analytical nanosensor based on graphene oxide/silver nanoparticles for simultaneous determination of quercetin and morin
    Yola, ML; Gupta, VK; Eren, T; Şen, AE; Atar, N
  • Molecular characterization and expression analysis of chalcone synthase gene during flower development in tree peony (Paeonia suffruticosa)
    Zhou, L; Wang, Y; Peng, Z

You’re reading a free preview. Subscribe to read the entire article.

Try 2 weeks free now

DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

or browse the journals available

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off

Sign up
for free
Start 14 day
Free Trial