Molecular detection of a putatively novel cyprinid herpesvirus in sichel ( Pelecus cultratus ) during a mass mortality event in Hungary

Molecular detection of a putatively novel cyprinid herpesvirus in sichel ( Pelecus cultratus )... In the early summer of 2014, mass mortality of sichel ( Pelecus cultratus ) was observed in Lake Balaton, Hungary. Histological examination revealed degenerative changes within the tubular epithelium, mainly in the distal tubules and collecting ducts in the kidneys and multifocal vacuolisation in the brain stem and cerebellum. Routine molecular investigations showed the presence of the DNA of an unknown alloherpesvirus in some specimens. Subsequently, three genes of the putative herpesviral genome (DNA polymerase, terminase, and helicase) were amplified and partially sequenced. A phylogenetic tree reconstruction based on the concatenated sequence of these three conserved genes implied that the virus belongs to the genus Cyprinivirus within the family Alloherpesviridae . The sequences of the sichel herpesvirus differ markedly from those of the cypriniviruses CyHV-1, CyHV-2 and CyHV-3, putatively representing a fifth species in the genus. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Molecular detection of a putatively novel cyprinid herpesvirus in sichel ( Pelecus cultratus ) during a mass mortality event in Hungary

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Publisher
Springer Vienna
Copyright
Copyright © 2015 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-015-2348-8
Publisher site
See Article on Publisher Site

References

  • The order Herpesvirales
    Davison, AJ; Eberle, R; Ehlers, B; Hayward, GS; McGeoch, DJ; Minson, AC; Pellett, PE; Roizman, B; Studdert, MJ; Thiry, E
  • Molecular confirmation of a new herpesvirus from catfish ( Ameiurus melas ) by testing the performance of a novel PCR method, designed to target the DNA polymerase gene of alloherpesviruses
    Doszpoly, A; Kovács, ER; Bovo, G; LaPatra, SE; Harrach, B; Benkő, M
  • Herpesviral haematopoietic necrosis of goldfish, Carassius auratus (L.)
    Jung, SJ; Miyazaki, T
  • TOPALi: software for automatic identification of recombinant sequences within DNA multiple alignments
    Milne, I; Wright, F; Rowe, G; Marshall, DF; Husmeier, D; McGuire, G

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