Molecular confirmation of a new herpesvirus from catfish ( Ameiurus melas ) by testing the performance of a novel PCR method, designed to target the DNA polymerase gene of alloherpesviruses

Molecular confirmation of a new herpesvirus from catfish ( Ameiurus melas ) by testing the... A PCR method with consensus degenerate primers was developed for the detection of herpesviruses (HVs) of anamnia. Compared to previously published PCRs, targeting the DNA polymerase gene of fish HVs, the size of PCR products was more than tripled. Although broad applicability of the method could not be proven, approximately 1,600-bp fragments from HVs of white sturgeon ( Acipenser transmontanus ) and black bullhead ( Ameiurus melas ) were obtained and sequenced. Phylogenetic tree reconstructions showed both HVs to be monophyletic with the single member (ictalurid HV-1) of the genus Ictalurivirus in the new family Alloherpesviridae . http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Molecular confirmation of a new herpesvirus from catfish ( Ameiurus melas ) by testing the performance of a novel PCR method, designed to target the DNA polymerase gene of alloherpesviruses

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Publisher
Springer Journals
Copyright
Copyright © 2008 by Springer-Verlag
Subject
Biomedicine; Infectious Diseases; Medical Microbiology ; Virology
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-008-0230-7
Publisher site
See Article on Publisher Site

Abstract

A PCR method with consensus degenerate primers was developed for the detection of herpesviruses (HVs) of anamnia. Compared to previously published PCRs, targeting the DNA polymerase gene of fish HVs, the size of PCR products was more than tripled. Although broad applicability of the method could not be proven, approximately 1,600-bp fragments from HVs of white sturgeon ( Acipenser transmontanus ) and black bullhead ( Ameiurus melas ) were obtained and sequenced. Phylogenetic tree reconstructions showed both HVs to be monophyletic with the single member (ictalurid HV-1) of the genus Ictalurivirus in the new family Alloherpesviridae .

Journal

Archives of VirologySpringer Journals

Published: Nov 1, 2008

References

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